Although enzymes are known for their excellent catalytic performance, industrial, medical or biotechnological applications should overcome some drawbacks like long-term stability under specific conditions of the application. Immobilized enzymes have offered advantages over soluble counterparts in many industrial and laboratory scale applications by increasing operational stability and reusability. When the immobilization matrix has magnetic properties, an additional advantage is obtained as simpler processing. Iron-based superparamagnetic nano-sized particles has large surface area for bio-compatible applications are especially in focus. Adding nanofibrous polymers to magnetic nanoparticles has been an excellent way to increase efficiency of biocatalyst immobilization by further increasing loading capacity. This chapter explains various magnetic enzyme-nanoparticles based preparations with potential for future industrial applications like invertase, lipase and as model studies and focus on the nanofibrous polymer brush grafting as a way to increase catalytic efficiency of magnetic nanoparticles.
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http://dx.doi.org/10.1016/bs.mie.2019.09.004 | DOI Listing |
Int J Biol Macromol
December 2024
International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China. Electronic address:
African swine fever has caused huge losses to the global pig industry. In the absence of effective vaccines, reliable detection methods are crucial. The p30 protein of ASFV is often used as a target for detection due to its high antigenicity in the early stage of virus replication.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
Department of Chemistry, Imam Khomeini International University, P.O. Box 288, Qazvin, Iran.
A novel nanocomposite magnetic hydrogel was synthesized based on κ-carrageenan, acrylic acid, and activated carbon as an absorbent for removing heavy metal ions from aqueous solution. FT-IR spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and vibration sample magnetometer (VSM) were employed to confirm the structure of the nanocomposite hydrogels. The effects of contact time, pH, particle size, temperature, and metal ion concentration on the metal ion adsorption were investigated.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
School of Environmental and Chemical Engineering, Xi'an Key Laboratory of Textile Chemical Engineering Auxiliaries, Engineering Research Center of Biological Resources Development and Pollution Control Universities of Shaanxi Province, Key Laboratory of Textile Dyeing Wastewater Treatment Universities of Shaanxi Province, Xi'an Polytechnic University, Xi'an 710048, PR China. Electronic address:
Improving the catalytic efficiency and recyclability of immobilized enzyme remained a serious challenge in industrial applications. Enzyme immobilization in the amorphous zeolite imidazolate framework (aZIF) preserved high enzyme activity, but still faced separation difficulties and a low catalytic efficiency in practice. In this study, a one-pot co-precipitation method was used to form the enzyme-aZIF/magnetic nanoparticle (MNP) biocomposite by rapidly precipitating snailase (Sna) and β-glucosidase (β-G) with metal/ligand on MNP and modifying with L-aspartic acid (Asp).
View Article and Find Full Text PDFMikrochim Acta
December 2024
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, 214122, China.
A dual-signal aptamer-based assay utilizing colorimetric and fluorescence techniques was developed for the determination of zearalenone (ZEN). The CdTe quantum dots, serving as the fluorescent signal source, were surface-modified onto FeO@SiO and subsequently functionalized with the aptamer. The COF-Au was modified with complementary chain, which possessed peroxide (POD)-like enzyme properties, and could catalyze the peroxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to ox TMB, resulting in the generation of colorimetric signals.
View Article and Find Full Text PDFThis manuscript describes the successful synthesis of FeO nanoparticles coated with β-cyclodextrin-intercalated layered double hydroxide, which were utilized to remove Uranium (VI) from an aqueous solution effectively. The newly developed nano-adsorbent underwent thorough analysis through advanced techniques such as scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), vibrating sample magnetometer (VSM), and energy-dispersive X-ray analysis (EDX). Through the utilization of a one-variable-at-a-time strategy, we effectively enhanced the removal process by optimizing key factors such as the sample's pH and the amount of adsorbent utilized.
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