Reported herein is the development of a user-friendly platform for simple and transformative automation based on standard HPLC equipment. We showcase how the improved platform works in application to the completely automated, a "press of the button," synthesis of various glycan sequences.
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http://dx.doi.org/10.1039/c9cc08876h | DOI Listing |
J Chromatogr A
January 2025
School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, PR China; Key Laboratory of Traditional Chinese Medicine Classical Theory, Ministry of Education, Shandong University of Traditional Chinese Medicine, Jinan 250355, PR China. Electronic address:
Countercurrent chromatography (CCC) is an efficient technique for purifying bioactive natural compounds, but selecting the solvent system can be a time-consuming and crucial process for successful separation. This paper discussed the HPLC-assisted mathematical prediction method for the n-hexane/alcohol solvents/water (HAWat) and ethyl acetate/n-butanol/water (EBuWat) systems and designed an intelligent online selection system to simplify the separation process. First, the applicable rage of HAWat and EBuWat solvent systems were quantified by the methanol concentration at the column inlet when template molecules peak in a HPLC analysis (B%).
View Article and Find Full Text PDFChem Commun (Camb)
January 2020
Department of Chemistry and Biochemistry, University of Missouri - St. Louis, One University Boulevard, St. Louis, Missouri 63121, USA.
Reported herein is the development of a user-friendly platform for simple and transformative automation based on standard HPLC equipment. We showcase how the improved platform works in application to the completely automated, a "press of the button," synthesis of various glycan sequences.
View Article and Find Full Text PDFJ Org Chem
October 2016
Department of Chemistry and Biochemistry, University of Missouri-St. Louis , One University Boulevard, St. Louis, Missouri 63121, United States.
The development of a useful methodology for simple, scalable, and transformative automation of oligosaccharide synthesis that easily interfaces with existing methods is reported. The automated synthesis can now be performed using accessible equipment where the reactants and reagents are delivered by the pump or the autosampler and the reactions can be monitored by the UV detector. The HPLC-based platform for automation is easy to setup and adapt to different systems and targets.
View Article and Find Full Text PDFOrg Lett
June 2012
Department of Chemistry and Biochemistry and the Center for Nanoscience, University of Missouri-St. Louis, One University Boulevard, St. Louis, Missouri 63121, USA.
A standard HPLC was adapted to polymer supported oligosaccharide synthesis. Solution-based reagents are delivered using a software-controlled solvent delivery system. The reaction progress and completion can be monitored in real time using a standard UV detector.
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