Elettra - Sincrotrone Trieste, Strada Statale 14 - km 163,5 in AREA Science Park, 34149 Basovizza, Trieste, Italy. Electronic address:
Published: March 2020
AI Article Synopsis
Article Abstract
This work presents a thorough study on the Amide III band in fibrous proteins using Fourier Transformed Infrared Spectroscopy (FTIR). Type I collagen was chosen as a model for this family of proteins, not only because of its important role in mammalian tissues, but also for its involvement in several pathologies. In order to disclose the conformational information contained in the collagen bands, the spectral characteristics of Amide III of type I collagen were related to the ones of Amide I band, performing experiments of thermal denaturation of the protein in acidic solution. Data acquired allowed to observe the protein unfolding and retrieve information about its structural arrangements during the thermal cycle. Taking as guideline the well-known behaviour of the Amide I band, we correlated the structural changes deducible from Amide I analysis with the ones detectable for Amide III band, by exploiting three spectral analysis techniques, namely 2D-correlation analysis, second derivative analysis, and peak-fitting. This approach enabled us to jointly support the obtained results and finally to assign the components of the Amide III of a typical fibrous protein, such as type I collagen, to its characteristic secondary structure.
Background And Objective: Breast-conserving surgery (BCS) plays a crucial role in breast cancer treatment, with a primary focus on ensuring cancer-free surgical margins, particularly for patients undergoing neoadjuvant treatment. After neoadjuvant treatment, tumor regression can complicate the differentiation between breast cancer and adjacent tissues. Raman spectroscopy, as a rapid and non-invasive optical technique, offers the advantage of providing detailed biochemical information and molecular signatures of internal molecular components in tissue samples.
Ru(II)-Catalyzed "On Water" direct aryl C(sp2)-H amidation of 2-arylbenzo[d]-thiazole/oxazole with acyl azide is reported under silver-free condition. Deuterium scrambling experiments suggested reversible C-H activation catalyzed by active cationic ruthenium species. The organic solvents such as DCE, DMF, DMSO, MeCN, dioxane, and PhMe were not conducive for the C-H amidation except for PhCl in which case, however, inferior yield (31%) was obtained.
The analgesic efficacy and safety of liposomal bupivacaine (LB) in third molar extraction was evaluated in this phase 3, double-blind, placebo-controlled study of subjects undergoing bilateral third molar extraction. Subjects were randomized 2: 1 to infiltration with LB (133 mg/10 mL) or placebo, and received opioid rescue medication as needed. Primary efficacy measure was cumulative area under the curve (AUC) of numeric rating scale (NRS) pain severity scores through 48 hours (AUC of NRS0-48) postsurgery.
Acute liver failure (ALF) is defined as the loss of hepatic function in conjunction with hepatic encephalopathy and coagulopathy. There is histological evidence of profound hepatocyte damage. If it is not aggressively managed, ALF can be fatal within a few days.
NH600001 is a new general anaesthetic drug with a structure similar to etomidate. The objective of this study was to investigate the relationship between concentrations of NH600001 and sedation efficacy based on data from phase I-II studies and factors influencing the pharmacokinetics and pharmacodynamics of NH600001. The dataset consisted of 2 phase I studies in healthy subjects and 1 phase II study in patients undergoing gastroscopy.
