OpenSFDI: an open-source guide for constructing a spatial frequency domain imaging system.

Matthew Applegate Kavon Karrobi Joseph Angelo Wyatt Austin Syeda Tabassum Enagnon Aguénounon Karissa Tilbury Rolf Saager Sylvain Gioux Darren Roblyer

J Biomed Opt

Boston Univ., United States.

Published: January 2020

: Spatial frequency domain imaging (SFDI) is a diffuse optical measurement technique that can quantify tissue optical absorption (μ) and reduced scattering () on a pixel-by-pixel basis. Measurements of μ at different wavelengths enable the extraction of molar concentrations of tissue chromophores over a wide field, providing a noncontact and label-free means to assess tissue viability, oxygenation, microarchitecture, and molecular content. We present here openSFDI: an open-source guide for building a low-cost, small-footprint, three-wavelength SFDI system capable of quantifying μ and as well as oxyhemoglobin and deoxyhemoglobin concentrations in biological tissue. The companion website provides a complete parts list along with detailed instructions for assembling the openSFDI system.

: We describe the design of openSFDI and report on the accuracy and precision of optical property extractions for three different systems fabricated according to the instructions on the openSFDI website.

: Accuracy was assessed by measuring nine tissue-simulating optical phantoms with a physiologically relevant range of μ and with the openSFDI systems and a commercial SFDI device. Precision was assessed by repeatedly measuring the same phantom over 1 h.

: The openSFDI systems had an error of 0 ± 6 % in μ and -2 ± 3 % in , compared to a commercial SFDI system. Bland-Altman analysis revealed the limits of agreement between the two systems to be ± 0.004 mm for μ and -0.06 to 0.1 mm for . The openSFDI system had low drift with an average standard deviation of 0.0007 mm and 0.05 mm in μ and , respectively.

: The openSFDI provides a customizable hardware platform for research groups seeking to utilize SFDI for quantitative diffuse optical imaging.

Download full-text PDF	Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7008504	PMC
http://dx.doi.org/10.1117/1.JBO.25.1.016002	DOI Listing

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