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A colorimetric method for determination of the prostate specific antigen based on enzyme-free cascaded signal amplification via  peptide-copper(II) nanoparticles. | LitMetric

A colorimetric method for determination of the prostate specific antigen based on enzyme-free cascaded signal amplification via  peptide-copper(II) nanoparticles.

Mikrochim Acta

Henan Province of Key Laboratory of New Optoelectronic Functional Materials, College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan, 455000, People's Republic of China.

Published: January 2020

AI Article Synopsis

Article Abstract

Biotinylated peptide-Cu nanoparticles (Cu-P NPs) were synthesized via "one-pot" self-assembly. The peptide P conststs of a hydrophobic dipeptide (FF), a tripeptide (KGH), and a biotin moiety attached to the terminal amino group of the Lys residue. The Cu-P NPs contain abundant catalytically active Cu ions which are liberated by acid-induced dissolution. The released Cu ions catalyze the oxidization of 3,3',5,5'-tetramethylbenzidine (TMB) by HO because of their intrinsic peroxidase activity, and this results in the formation of a blue-green coloration. Based on the streptavidin-biotin interaction, the Cu-P NPs were employed to establish an enzyme-free colorimetric method for determination of prostate-specific antigen (PSA) as a model biomarker. Under optimal conditions, the linear response range is 0.001-1 ng mL, with a limit of detection as low as 1 pg mL. Graphical abstract Schematic illustration of a colorimetric immunoassay for the prostate specific antigen (PSA) with biotinylated peptide-Cu nanoparticle (Cu-P NP) as the signal label based on the streptavidin (SA)-biotin interaction. The signal was produced by Cu-catalyzed oxidization of 3,3',5,5'-tetramethylbenzidine (TMB). P: KGHFF.

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http://dx.doi.org/10.1007/s00604-019-4074-5DOI Listing

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