Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6976797PMC
http://dx.doi.org/10.1016/j.bpj.2019.12.032DOI Listing

Publication Analysis

Top Keywords

dynamics membrane-bound
4
membrane-bound g12v-kras
4
g12v-kras simulations
4
simulations single-molecule
4
single-molecule fret
4
fret native
4
native nanodiscs
4
dynamics
1
g12v-kras
1
simulations
1

Similar Publications

Article Synopsis
  • The transmembrane potential is crucial for cellular functions like signaling and energy production, with Rhodamine voltage reporters (RhoVRs) serving as small, non-invasive sensors that can detect voltage changes, especially in mitochondria.
  • Extensive simulations and free-energy calculations revealed that the orientation of RhoVRs relative to membranes, influenced by their polarized functional groups, significantly impacts their voltage sensitivity and localization within cells.
  • The study's findings on the relationship between the chemical structure of RhoVRs and their membrane behavior offer valuable insights for designing fluorescent dyes that better detect voltage changes.
View Article and Find Full Text PDF

Adoptive cell therapies (ACT) have shown reduced efficacy against solid tumor malignancies compared to hematologic malignancies, partly due to the immunosuppressive nature of the tumor microenvironment (TME). ACT efficacy may be enhanced with pleiotropic cytokines that remodel the TME; however, their expression needs to be tightly controlled to avoid systemic toxicities. Here we show T cells can be armored with membrane-bound cytokines with surface expression regulated using drug-responsive domains (DRDs) developed from the 260-amino acid protein human carbonic anhydrase 2 (CA2).

View Article and Find Full Text PDF

DOPAC as a modulator of α-Synuclein and E46K interactions with membrane: Insights into binding dynamics.

Int J Biol Macromol

January 2025

Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy. Electronic address:

α-Synuclein (Syn) is an intrinsically disordered protein, abundant in presynaptic neurons. It is a constituent of the Lewis Body inclusions as amyloid fibrils, in Parkinson's disease patients. It populates an ensemble of conformations and floats between the free random coil and the membrane-bound α-helical species.

View Article and Find Full Text PDF

An exchangeable SIM probe for monitoring organellar dynamics of necrosis cells and intracellular water heterogeneity in kidney repair.

Proc Natl Acad Sci U S A

January 2025

Institute of Optical Materials and Chemical Biology, Guangxi Key Laboratory of Electrochemical Energy Materials, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, People's Republic of China.

Monitoring subcellular organelle dynamics in real time and precisely assessing membrane heterogeneity in living cells are very important for studying fundamental biological mechanisms and gaining a comprehensive understanding of cellular processes. However, there remains a shortage of effective tools for these purposes. Herein, we propose a strategy to develop the exchangeable water-sensing probeAPBD for time-lapse imaging of dynamics in cellular membrane-bound organelle morphology with structured illumination microscopy at the nanoscale.

View Article and Find Full Text PDF

Exploring the role of exosomal lncRNA in cancer immunopathogenesis: Unraveling the immune response and EMT pathways.

Exp Cell Res

December 2024

Medical Laboratory Technique College, The Islamic University, Najaf, Iraq; Medical Laboratory Technique College, The Islamic University of Al Diwaniyah, Al Diwaniyah, Iraq; Medical Laboratory Technique College, The Islamic University of Babylon, Babylon, Iraq. Electronic address:

Exosomes are membrane-bound vesicles secreted by diverse cell types, serving as crucial mediators in intercellular communication and significantly influencing cancer development. Exosomes facilitate complex signaling processes in the tumor microenvironment for immunomodulation, metastasis, angiogenesis, and treatment resistance. Notably, long non-coding RNAs (lncRNAs), a class of non-coding RNAs, engage with mRNA, DNA, proteins, and miRNAs to modulate gene expression through multiple mechanisms, including transcriptional, post-transcriptional, translational, and epigenetic pathways.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!