Evaluating bioactive constituents and toxicological effects of aqueous extract of fermented seeds in rats.

Objective: This study aimed at evaluating the bioactive constituents and the toxicological profile of the aqueous fermented seed extract of .

Materials And Methods: The chemical constituents of fermented were assessed using GC-MS. For acute toxicity study, one-time doses of up to 5000 mg/kg of the extract were orally administered to male and female rats whereas 200, 400 and 800 mg/kg of the extract were orally administered daily for 14 days in sub-acute toxicity investigation. Biochemical, haematological and lipid profiles were assessed following standard methods.

Results: Bioactive compounds such as citronellol and oxirane, tetradecyl- (hexadecylene oxide) were identified in the extract. In acute toxicity test, no death or sign of toxicity was identified. For sub-acute study, ALT decreased significantly (p<0.05) while HDL-C had dose-dependent increases. No effect (p<0.05) on haematological parameters except on platelets was found. No histopathological changes were observed.

Conclusion: Our results demonstrated that the extract of fermented caused no toxic effects in the rats at the tested doses. Therefore, they may be termed safe for consumption and therapeutic uses.