An enhanced cathodic electrochemiluminescence (ECL) assay for prostate-specific antigen (PSA) is developed based on the in situ activation of a semiconductor nanomaterial. An excellent ECL emitter (CdS/TiO nanotubes) was fabricated by the combination of TiO nanotubes (NTs) and thioglycolic acid-capped CdS nanocrystals (NCs). After the activation of the hydrogen peroxide-citric acid solution, the ECL signal was enhanced 265 times compared with that of the original TiO NT with HO as co-reactant. For the ECL assay, activated CdS/TiO NTs were assembled with complementary DNA, PSA aptamer and probe DNA-functionalized SiO@Pt nanoparticles (NPs) via DNA hybridization to form the detection platform. The SiO@Pt NPs acted as ECL quencher of CdS/TiO NTs. In the presence of PSA, ECL increased after the release of pDNA-SiO@Pt NPs because of the binding of PSA to the aptamer. An "off-on" ECL phenomenon appeared. The enhanced ECL signals were used for sensitive determination of PSA. The dynamic range was 0.001 to 50 ng mL with a detection limit of 0.4 pg mL (S/N = 3). This new approach conceivably paves the way for fabricating various other enhanced ECL emitter systems, with good application prospects in clinical practice. Graphical abstract The activated CdS/TiO nanotubes and SiO@Pt nanoparticles were synthesized and used to develop an energy-transfer electrochemiluminescence analysis method with high sensitivity and anti-interference performance.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00216-019-02365-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Energy-Transfer-Based Dual-Mode PEC-ECL Biosensor for Acetamiprid Analysis Sensitized by Two-Step DNA Circuit Amplification.
ACS Appl Mater Interfaces
January 2025
School of Chemistry and Materials Science, Jiangsu Normal University, Xuzhou 221116, China.
Sensitive and accurate determination of acetamiprid is highly desirable for guaranteeing food safety. In this Letter, an energy-transfer-based dual-mode biosensor was developed using zinc-based metal-organic frameworks (Zn-MOFs) acting as both photoelectrochemical (PEC) and electrochemiluminescent (ECL) donors and Pt@CuO cubic nanocrystals (CNs) as the energy acceptor for detecting acetamiprid. By integration of aptamer recognition with two-step DNA circuit amplification (entropy-driven DNA cycle and DNA walker), the detection of acetamiprid was converted into the assay of abundant intermediate DNA strands.
View Article and Find Full Text PDFPositional training demands in the English Premier League and English Championship. A longitudinal study across consecutive seasons.
Biol Sport
January 2025
Sports Science School of Rio Maior - Instituto Politecnico de Santarem, 2040-413 Rio Maior, Santarém District, Santarém, Portugal.
The aims of this study were to: compare training loads between the English Premier League (EPL) and English Championship League (ECL) and examine differences between playing positions. Forty-six 1 team players from the same club participated in the study. GPS metrics were obtained during all EPL and ECL training sessions across four consecutive seasons, 2019-20 to 2022-23.
View Article and Find Full Text PDFPhysical match performance of elite soccer players from the English Championship League and the English Premier League: The effects of opponent ranking and positional differences.
Biol Sport
January 2025
Research Centre in Sports Sciences, Health and Human Development, 5001-801 Vila Real, Portugal.
This study aimed to examine physical match performance and the effects of opponent ranking and positional differences in both the English Championship League (ECL) and the English Premier League (EPL) over five consecutive seasons. Fifty-four professional outfield soccer players (average age 24.6 ± 5.
View Article and Find Full Text PDFFlower-like tailored carbon nitride oligomer as an excellent aggregation-induced electrochemiluminescence emitter for sensitive immunoassay of neuron-specific enolase via dual quenching by bimetallic phenolic networks.
J Colloid Interface Sci
January 2025
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. Electronic address:
The adjustment of the electrochemiluminescence (ECL) of polymeric carbon nitride (CN) is essential for its application in sensitive immunoassays. However, such modification through aggregation-induced emission (AIE) has not yet been reported. Herein, aggregation-induced ECL in CN oligomer (CNO) was induced through the introduction of a rotatable imine moiety, with the resulting material exhibiting excellent performance in the targeted immunodetection of neuron-specific enolase.
View Article and Find Full Text PDFMesoporous carbon nanospheres-assisted amplified electrochemiluminescence for L-cysteine detection.
Anal Biochem
January 2025
Key Laboratory of Green and Precise Synthetic Chemistry and Applications, Ministry of Education, Anhui Provincial Key Laboratory of Synthetic Chemistry and Applications, College of Chemistry and Materials Science, Huaibei Normal University, Huaibei, Anhui 235000, PR China. Electronic address:
Luminol-loaded mesoporous carbon nanospheres (MCs@LU) were utilized to develop a highly sensitive electrochemiluminescence (ECL) sensor for the detection of L-cysteine (L-Cys). L-Cys acted as the coreactant of luminol, and the pore confinement effect of mesoporous carbons (MCs) resulted in a robust ECL signal. Upon optimization, a linear correlation between the ECL intensity and L-Cys concentration was observed over the range of 5.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!