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Centromeres are maintained epigenetically by the presence of CENP-A, an evolutionarily conserved histone H3 variant, which directs kinetochore assembly and hence centromere function. To identify factors that promote assembly of CENP-A chromatin, we affinity-selected solubilized fission yeast CENP-A chromatin. All subunits of the Ino80 complex were enriched, including the auxiliary subunit Hap2. Chromatin association of Hap2 is Ies4-dependent. In addition to a role in maintenance of CENP-A chromatin integrity at endogenous centromeres, Hap2 is required for de novo assembly of CENP-A chromatin on naïve centromere DNA and promotes H3 turnover on centromere regions and other loci prone to CENP-A deposition. Prior to CENP-A chromatin assembly, Hap2 facilitates transcription from centromere DNA. These analyses suggest that Hap2-Ino80 destabilizes H3 nucleosomes on centromere DNA through transcription-coupled histone H3 turnover, driving the replacement of resident H3 nucleosomes with CENP-A nucleosomes. These inherent properties define centromere DNA by directing a program that mediates CENP-A assembly on appropriate sequences.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7000912 | PMC |
| http://dx.doi.org/10.1101/gad.332536.119 | DOI Listing |
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Article Synopsis
- Centromeres rely on the histone variant CENP-A and the role of surrounding DNA repeats is not fully understood, while retroelements are abundant in centromeres and may help with transcription and CENP-A integration.* -
- This study focuses on the retroelement Jockey-3 in Drosophila melanogaster, showing it is a significant part of the centromeric transcriptome and that its RNA localizes to centromeres during cell division.* -
- The research suggests that Jockey-3 inserts itself at centromeres to aid its own replication, while also supporting transcription in these areas, which could provide insights into similar mechanisms in other species.*
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