Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7264358 | PMC |
| http://dx.doi.org/10.1038/s41422-019-0270-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
RNA m6A methylation across the transcriptome.
Mol Cell
February 2023
Ludwig Institute for Cancer Research, University of Oxford, Roosevelt Dr, Headington, Oxford OX3 7DQ, UK. Electronic address:
Since the early days of foundational studies of nucleic acids, many chemical moieties have been discovered to decorate RNA and DNA in diverse organisms. In mammalian cells, one of these chemical modifications, N6-methyl adenosine (m6A), is unique in a way that it is highly abundant not only on RNA polymerase II (RNAPII) transcribed, protein-coding transcripts but also on non-coding RNAs, such as ribosomal RNAs and snRNAs, mediated by distinct, evolutionarily conserved enzymes. Here, we review RNA m6A modification in the light of the recent appreciation of nuclear roles for m6A in regulating chromatin states and gene expression, as well as the recent discoveries of the evolutionarily conserved methyltransferases, which catalyze methylation of adenosine on diverse sets of RNAs.
View Article and Find Full Text PDFStructural insights into molecular mechanism for N-adenosine methylation by MT-A70 family methyltransferase METTL4.
Nat Commun
September 2022
State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry and Biophysics, Institute of Plant Biology, School of Life Sciences, Fudan University, 200438, Shanghai, China.
Article Synopsis
- METTL4 is a methyltransferase in Arabidopsis that mediates the methylation of RNA and DNA, specifically functioning as a U2 snRNA MTase for N-2'-O-dimethyladenosine (mAm) which affects flowering time.
- The study reveals that METTL4 catalyzes the N-methylation of 2'-O-methyladenosine (Am) in vitro, using structural insights that show its unique binding cavity and catalytic center crucial for substrate interaction.
- Comparisons with other methyltransferases suggest that METTL4 shares a similar catalytic mechanism with the mRNA m6A enzyme complex (METTL3/METTL14), highlighting a potential commonality among
N-6-Methyladenosine in Vasoactive microRNAs during Hypoxia; A Novel Role for METTL4.
Int J Mol Sci
January 2022
Department of Surgery, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.
N-6-methyladenosine (m6A) is the most prevalent post-transcriptional RNA modification in eukaryotic cells. The modification is reversible and can be dynamically regulated by writer and eraser enzymes. Alteration in the levels of these enzymes can lead to changes in mRNA stability, alternative splicing or microRNA processing, depending on the m6A-binding proteins.
View Article and Find Full Text PDFMETTL4 catalyzes m6Am methylation in U2 snRNA to regulate pre-mRNA splicing.
Nucleic Acids Res
September 2020
Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672, Singapore.
Article Synopsis
- N 6-methylation of 2'-O-methyladenosine (Am) in RNA leads to the formation of N6,2'-O-dimethyladenosine (m6Am), primarily involved in RNA processing in eukaryotic cells.
- The methyltransferase METTL4 has been identified as the key enzyme responsible for adding methyl groups to Am at position 30 in U2 snRNA, which is important for splicing regulation.
- Lack of METTL4 activity results in the absence of m6Am in U2 snRNA, leading to altered splicing events in pre-mRNA transcripts, especially those with specific splice site weaknesses.
CG14906 (mettl4) mediates mA methylation of U2 snRNA in .
Cell Discov
June 2020
Department of Medicine, Division of Newborn Medicine and Epigenetics Programe, Boston Children's Hospital, Boston, MA 02115 USA.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!