Study Design: Retrospective cohort study.
Objectives: Doubts still remain whether a sufficient reduction of vertebral body fractures can be achieved if a percutaneous surgical approach is adopted. The aim of this retrospective cohort study was to analyze the reduction and perioperative complications using a percutaneous versus open surgical approach for dorsal stabilization.
Methods: Patients suffering from a traumatic injury of the thoracic or lumbar spine who were treated with dorsal stabilization at our level 1 trauma center were included. From January 2007 through June 2009, only the open approach was used; from June 2009 through March 2014, only the percutaneous approach with a special reduction technique was used. Retrospectively, total reduction, perioperative and intraoperative data, and postoperative complications were analyzed.
Results: Using the percutaneous (n=185) versus open surgical approach (n=91), the mean reduction over all spinal injuries was 7.7±6.6 versus 8.3±5.5 degrees, respectively. The mean operation time was 75.5±50.2 versus 71.5±41.9 minutes, respectively. There was no significant difference between the 2 treatment groups in terms of the total reduction (P=0.753) or operation time (P=0.814).After the percutaneous and open surgical approach, 7 (3.8%) and 22 (24.2%) complications were documented. Complications requiring interventions were more frequent in the open treatment group.
Conclusions: Percutaneous dorsal stabilization seems to be a good alternative to the traditional open surgical approach. The fracture's reduction is similar, whereas severe complications are reduced and the operation time is not prolonged.
Level Of Evidence: Level III-retrospective cohort study.
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http://dx.doi.org/10.1097/BSD.0000000000000925 | DOI Listing |
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Division of Gastroenterology & Hepatology, University of Toronto, Toronto, ON, Canada.
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Department of Immunology, Genetics and Pathology, Uppsala University, 75185 Uppsala, Sweden; Department of Surgical Sciences, Uppsala University, 75185 Uppsala, Sweden. Electronic address:
Here, we present a protocol for guiding tissue preparation and flow cytometric analysis in subcutaneous murine tumor models and secondary lymphoid organs. We describe steps for dissociating tumors, spleens, and lymph nodes to obtain single-cell suspensions. We then detail procedures for immune cell staining and analysis and gating strategies including the use of fluorescence-minus-one controls (FMOs).
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