The activation energy of thermodenaturation of erythrocyte spectrin was defined by two different methods as Esp = 218 +/- 4 kcal/mole. This value was more than two times greater than that of thermohemolysis activation energy (Eth = 99 +/- 5 kcal/mole). The conclusion is made that spectrin does not play a significant role in thermohemolysis of erythrocytes.
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