AI Article Synopsis

  • NAT1 (N-acetyltransferase 1) is linked to poor survival rates in breast cancer patients and influences cancer cell behavior.
  • NAT1 deletion in cell lines led to increased adhesion to collagen, but did not affect cell migration.
  • The loss of NAT1 reduced invasion and altered cell shape, suggesting its role in regulating adhesion and invasion through integrin ITGαV expression.

Article Abstract

Reducted arylamine N-acetyltransferase (NAT1) in breast cancers is associated with poor patient survival. NAT1 has also been associated with changes in cancer cell survival and invasion both and . Here, we report the effects of NAT1 in cancer cell invasion by addressing its role in adherence, migration, and invasion . The NAT1 gene was deleted in MDA-MB-231, HT-29 and HeLa cells using CRISPR/Cas9 gene editing. Loss of NAT1 increased adherence to collagen in all three cell-lines but migration was unaffected. NAT1 deletion decreased invasion and induced changes to cell morphology. These effects were independent of matrix metalloproteinases but were related to integrin ITGαV expression. The data suggest NAT1 is important in adhesion and invasion through integrin expression.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961680PMC
http://dx.doi.org/10.1080/19336918.2019.1710015DOI Listing

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