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An optofluidic "tweeze-and-drag" cell stretcher in a microfluidic channel. | LitMetric

An optofluidic "tweeze-and-drag" cell stretcher in a microfluidic channel.

Lab Chip

Photonic Device Laboratory, Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong SAR, China.

Published: February 2020

AI Article Synopsis

Article Abstract

The mechanical properties of biological cells are utilized as an inherent, label-free biomarker to indicate physiological and pathological changes of cells. Although various optical and microfluidic techniques have been developed for cell mechanical characterization, there is still a strong demand for non-contact and continuous methods. Here, by combining optical and microfluidic techniques in a single desktop platform, we demonstrate an optofluidic cell stretcher based on a "tweeze-and-drag" mechanism using a periodically chopped, tightly focused laser beam as an optical tweezer to trap a cell temporarily and a flow-induced drag force to stretch the cell in a microfluidic channel transverse to the tweezer. Our method leverages the advantages of non-contact optical forces and a microfluidic flow for both cell stretching and continuous cell delivery. We demonstrate the stretcher for mechanical characterization of rabbit red blood cells (RBCs), with a throughput of ∼1 cell per s at a flow rate of 2.5 μl h at a continuous-wave laser power of ∼25 mW at a wavelength of 1064 nm (chopped at 2 Hz). We estimate the spring constant of RBCs to be ∼14.9 μN m. Using the stretcher, we distinguish healthy RBCs and RBCs treated with glutaraldehyde at concentrations of 5 × 10% to 2.5 × 10%, with a strain-to-concentration sensitivity of ∼-1529. By increasing the optical power to ∼45 mW, we demonstrate cell-stretching under a higher flow rate of 4 μl h, with a higher throughput of ∼1.5 cells per s and a higher sensitivity of ∼-2457. Our technique shows promise for applications in the fields of healthcare monitoring and biomechanical studies.

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Source
http://dx.doi.org/10.1039/c9lc01026bDOI Listing

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