The activity of NAD(P)-dependent mitochondrial malic enzyme was considerably inhibited by KCN, whereas under the same conditions azide affected the enzyme only slightly. Kinetic studies showed that KCN is an uncompetitive inhibitor of mitochondrial malic enzyme from human placenta. In contrast to the mitochondrial enzyme, the cytosolic malic enzyme was only slightly affected by KCN and under the same conditions the effect of azide was negligible. The effect of KCN was compared to this on the malic enzyme from other sources.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Trade-Off Between Enzymatic Antioxidant Defense and Accumulation of Organic Metabolite Affects Salt Tolerance of White Clover Associated with Redox, Water, and Metabolic Homeostases.
Plants (Basel)
January 2025
Department of Turf Science and Engineering, College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
White clover () is an excellent perennial cold-season ground-cover plant for municipal landscaping and urban greening. It is, therefore, widely distributed and utilized throughout the world. However, poor salt tolerance greatly limits its promotion and application.
View Article and Find Full Text PDFComparative Transcriptome Analysis Reveals Potential Molecular Regulation of Organic Acid Metabolism During Fruit Development in Late-Maturing Hybrid Citrus Varieties.
Int J Mol Sci
January 2025
Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu 611130, China.
Late-maturing hybrid citrus is a significant fruit that combines the best traits of both parents and is highly prized for its unique flavor. Not only can organic acids alter the flavor of citrus pulp, but they are also essential for cellular metabolism, energy conversion, and maintaining the acidbase balance in plant tissues. Although organic acids play a key role in the quality formation of citrus fruits, there is still insufficient research on the metabolic processes of organic acids in late-maturing hybrid citrus varieties.
View Article and Find Full Text PDFEffects of glycosidases and GSH pretreatments, fermentation temperatures, and aging time on the physicochemical, organic acids, and aroma profiles of perry.
Food Res Int
February 2025
Shandong University of Science and Technology, Qingdao 266590, China. Electronic address:
The lack of sufficient flavour in perry represents a barrier to its further industrialization. This study aimed to investigate the effects of glutathione (GSH), β-glucosidase (Glu), and α-L-rhamnosidase (Rha) pretreatments, the fermentation temperature from 16 °C to 28 °C, and the aging time of 1, 2, and 3 years (PA1, PA2, and PA3) on the physicochemical properties, organic acids, and aroma profiles were investigated. The results demonstrated that the synergistic effect of Glu, Rha, and GSH was more effective than their individual or paired applications in enhancing the varietal aromas.
View Article and Find Full Text PDFDihydroporphyrin iron (III) enhances low temperature tolerance by increasing carbon and nitrogen metabolism in .
Front Plant Sci
January 2025
National Center for Traditional Chinese Medicine (TCM) Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, China.
Dihydroporphyrin iron (DH-Fe) is a novel plant growth regulator that plays significant roles in plant stress resistance. We found that is extremely sensitive to low temperature (LT) with a threshold of 25°C. To evaluate whether and how DH-Fe alleviates LT stress in , different DH-Fe concentrations (0, 10, 20, and 40 μg·L) were applied to estimate its effects on C and N metabolism and antioxidative capacity in grown under 20°C.
View Article and Find Full Text PDFA novel lncRNA PDG1 targets NADP-ME to modulate TCA cycle and JH in Aspongopus chinensis diapause.
Int J Biol Macromol
January 2025
Institute of Entomology, Guizhou University, Guiyang 550025, PR China. Electronic address:
Diapause is essential for insect survival under adverse environmental conditions and plays a crucial role in regulating reproduction. However, the role of long non-coding RNAs (lncRNAs) in this process remains unclear. In this study, we investigated the function of lncRNAs in the diapause of Aspongopus chinensis.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!