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tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging. | LitMetric
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tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging.

Daniel J Nieves Geva Hilzenrat Jason Tran Zhengmin Yang Hugh H MacRae Matthew A B Baker J Justin Gooding Katharina Gaus

R Soc Open Sci

EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia.

Published: December 2019

Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA-docking strands and application of SMLM via DNA-PAINT is demonstrated. tagPAINT is then used for T-cell receptor signalling proteins at the immune synapse as a proof of principle.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6936279PMC
http://dx.doi.org/10.1098/rsos.191268DOI Listing

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