The present study investigated aberrant methylation in colorectal cancer (CRC) and its impact on characteristics and prognosis of patients with CRC. Bone morphogenetic protein 2 () was identified as a target gene in oligonucleotide microarray expression profiling in a previous study. Subsequently, the methylation status was assessed in 498 patients with stage I-III CRC using methylation-specific polymerase chain reaction, and the association between methylation status, patient characteristics and prognosis was assessed. methylation was observed in 302/498 (60.6%) patients and was associated with positive lymph nodes and venous invasion (P<0.05). In the stage III subgroup, overall survival (OS) was significantly worse in the methylated group compared with in the unmethylated group (P=0.012). methylation was identified as an independent factor for poor OS in stage III patients (P=0.041). Notably, in the left-sided stage III CRC subgroup, relapse-free survival and OS were significantly worse in the methylated group than in the unmethylated group (P=0.048 and P=0.031, respectively). In conclusion, DNA hypermethylation of was a poor prognostic factor in patients with stage III disease, particularly in those with left-sided stage III CRC. methylation may be a biomarker for prognosis prediction and treatment decision-making.
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| http://dx.doi.org/10.3892/ol.2019.11091 | DOI Listing |
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