Premise: Microsatellite primers were developed for (Bromeliaceae), an endangered species distributed in the Andean Mountains of Bolivia and Peru.
Methods And Results: Genome skimming of , , and resulted in the selection of 46 pairs of cross-species microsatellite markers. Of these, 12 microsatellite primer pairs produced clear and polymorphic bands in . These primer sets were then used for the detection of potential polymorphisms in 84 individuals collected from four populations in Peru. The number of alleles per locus ranged from one to six, and the observed and expected levels of heterozygosity ranged from 0.000 to 0.8929 and from 0.000 to 0.7662, respectively.
Conclusions: The microsatellite markers developed in this study will be useful for future population genetic analyses and breeding system studies in .
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http://dx.doi.org/10.1002/aps3.11308 | DOI Listing |
Pathogens
January 2025
U.S. Geological Survey, Pennsylvania Cooperative Fish and Wildlife Research Unit, 403 Forest Resources Building, The Pennsylvania State University, University Park, PA 16802, USA.
In white-tailed deer (), closely related females form social groups, avoiding other social groups. Consequently, females infected with chronic wasting disease (CWD) are more likely to infect social group members. Culling has been used to reduce CWD transmission in high-risk areas; however, its effectiveness in removing related individuals has not been assessed.
View Article and Find Full Text PDFGenes (Basel)
January 2025
Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju 55365, Republic of Korea.
Background/objectives: Chrysanthemum (), a key ornamental and medicinal plant, presents challenges in cultivar identification due to high phenotypic similarity and environmental influences. This study assessed the genetic diversity and discrimination of 126 spray-type chrysanthemum cultivars.
Methods: About twenty-three simple sequence repeat (SSR) markers were screened for the discrimination of 126 cultivars, among which six SSR markers showed polymorphic fragments.
Genes (Basel)
January 2025
State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Background/objectives: The Pacific abalone originated in cold waters and is an economically important aquaculture shellfish in China. Our goal was to clarify the current status of the genetic structure of Pacific abalone in China.
Methods: In this study, eighteen polymorphic EST-SSR loci were successfully developed based on the hemolymph transcriptome data of Pacific abalone, and thirteen highly polymorphic EST-SSR loci were selected for the genetic variation analysis of the six populations collected.
Genes (Basel)
December 2024
Australian Centre for Ancient DNA, The Environment Institute, School of Biological Sciences, The University of Adelaide, Adelaide, SA 5000, Australia.
Unlabelled: In many human rights and criminal contexts, skeletal remains are often the only available samples, and they present a significant challenge for forensic DNA profiling due to DNA degradation. Ancient DNA methods, particularly capture hybridization enrichment, have been proposed for dealing with severely degraded bones, given their capacity to yield results in ancient remains.
Background/objectives: This paper aims to test the efficacy of genome-wide capture enrichment on degraded forensic human remains compared to autosomal STRs analysis.
Animals (Basel)
January 2025
Faculty of Fisheries Life Sciences, Pukyong National University, Nam-Gu, Busan 48513, Republic of Korea.
Microsatellite markers are widely used in aquaculture for genetic analysis and breeding programs, but challenges such as segregation distortion and allelic instability can impact their effectiveness in parentage verification and inheritance studies. This study evaluated 15 microsatellite loci in seven experimental olive flounder () families bred through 1:1 full-sibling crosses, assessing their utility for accurate parentage and inheritance stability. Parentage assignments were conducted within an expanded pool of 647 candidate parents (including the actual 14 parents), encompassing both closely related and moderately distant individuals.
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