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Premise: Microsatellite markers were developed for Labrador tea (, Ericaceae) to facilitate downstream genetic investigation of this species and the extremely closely related, circumboreal subsect. .

Methods And Results: Forty-eight primer pairs were designed using Illumina data and screened for excellent amplification. Sixteen successful pairs were developed as microsatellite markers using fluorescently labeled amplification to generate chromatogram data. These data were evaluated for intrapopulation and interpopulation variability in three populations from Alaska and Maine, USA, and the Northwest Territories, Canada. Fourteen polymorphic markers genotyped reliably, each with one to eight alleles. Cluster analysis indicates that across the range, populations can be easily discriminated. Cross-amplification in other subsect. species shows broad application of the developed markers within this small, well-supported clade.

Conclusions: These microsatellite markers exhibit significant variability and will be useful in population genetics within and for investigation of species boundaries across subsect. .

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923708PMC
http://dx.doi.org/10.1002/aps3.11306DOI Listing

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