Detection of specific antibody in swine oral fluids.

is a leading cause of bacterial foodborne-related illness and pork products are a food-associated source. With > 50% of U.S. swine herds testing positive for , asymptomatic carrier pigs that shed in their feces are a food safety and environmental contamination issue. Herd level surveillance of shedding status is useful, but collection of feces and culture methods for detection are laborious and time-consuming. Surveillance for -exposure through detection of -specific serum antibody is a reliable method, but presents labor and animal-welfare issues. Oral fluids are a reliable, antemortem sample with proven utility for surveillance in the swine industry. We tested oral fluid samples as a potential non-invasive, repeatable sample type for the presence of -specific antibodies. An indirect enzyme-linked immunosorbent assay (ELISA) detected anti- IgG, IgM, and predominantly IgA in oral fluids from serovar Typhimurium-exposed pigs. Furthermore, with minor modifications, a commercial ELISA-based kit also detected -specific antibodies in oral fluids. Collectively, oral fluids may serve as a prospective surveillance tool for herd level monitoring of exposure.