It has been reported that PA3 has an ability to absorb exogenous purines in the intestine to reduce a risk of gout and hyperuricemia. However, influences of this strain on gut microbiota and their metabolisms remain unclear. Herein, we aimed to investigate the effect of PA3 on microbiota composition and metabolisms. PA3 was isolated from yogurt and supplemented into a single-stage colonic fermentation in a culture volume of 30 ml and subjected to in vitro colonic simulation for 8 days. Microbiota composition was determined with 16S rRNA (V3 + V4) sequencing, and their metabolisms were predicted by PICRUSt. Short-chain fatty acids were measured by GC-MS. We found that PA3 reduced the diversity of microbiota, increased the relative abundances of (73.5%) and (36.5%), and decreased and . Total amount of short-chain fatty acids was found to decline. Fundamental metabolisms, especially nucleotide, was significantly higher after intervention with PA3, but the purine metabolism was lower, which means that PA3 might reduce uric acid concentrations by weakening purine metabolism. Our results indicated that PA3 can survive and play a role in the ascending colon environment. Therefore, the evaluation of the effect of PA3 on intestinal microbes and their metabolisms has great guiding significance for the development of treatment to prevent gout.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6924308PMC
http://dx.doi.org/10.1002/fsn3.1236DOI Listing

Publication Analysis

Top Keywords

pa3
9
gut microbiota
8
vitro colonic
8
colonic simulation
8
microbiota composition
8
short-chain fatty
8
fatty acids
8
purine metabolism
8
microbiota
5
metabolisms
5

Similar Publications

Multi-species tests in bioassays offer a holistic view of the ecosystem's response to toxicity, as different species display varying sensitivities to pollutants. This research aimed to assess the ability of toxicity tests' to distinguish contamination levels, examine site-specific effects, and investigate seasonal variability. Using a multispecies approach (Nannochloropsis oceanica, Artemia franciscana, and Arbacia nigra), bioassays evaluated marine water quality from Callao Bay in Peru across four sampling areas (Naval School: PA1, Peruvian Marine Institute: PA2, Callao Pier: PA3, and San Lorenzo Island: PA4).

View Article and Find Full Text PDF

Antibiotic resistance is a world wide problem mainly in developing countries. In this work, coelomic fluid (PCF) and paste (PBP) of Pheretima posthuma was assessed for its potential as antibiofilm and anti-quorum sensing (QS) agent against pathogenic bacterial biofilms. PCF and PBP were extracted and biofilm formation time kinetics was examined using crystal violet staining method by utilizing four bacterial isolates in bispecies biofilm (06 combinations; MH5-MH10) and multi species biofilms (05 combinations; MH11-MH15).

View Article and Find Full Text PDF
Article Synopsis
  • A study investigated the antibacterial effects of certain plant extracts, both individually and in combination with antibiotics, against multidrug-resistant (MDR) bacteria, a major health threat due to its resistance to conventional treatments.
  • Qualitative and quantitative phytochemical analyses revealed that the extracts contained phenols and flavonoids, with one extract showing the highest concentration of phenolic compounds and the other the highest flavonoid content.
  • The research found that the extracts exhibited antibacterial properties, with the best performing extract enhancing the effectiveness of several antibiotics, suggesting potential for developing natural treatments for MDR infections.
View Article and Find Full Text PDF

Chili anthracnose, caused by , causes significant yield loss in chili production. In this study, we investigated the elicitor properties of a rhamnolipid (RL)-enriched PA3 fraction derived from SWUC02 in inducing systemic resistance in yellow chili seedlings and antifungal activity against CFPL01 (Col). Fractionation of the ethyl acetate extract yielded 12 fractions, with PA3 demonstrating the most effective disease suppression, reducing the disease severity index to 4 ± 7.

View Article and Find Full Text PDF
Article Synopsis
  • Rare-earth metal-organic frameworks (RE-MOFs) with fluoride donors have complex structures that are key for their practical applications.
  • The framework Y-ndc--MOF shows structural disorders and specific guest positions, influenced by the types of guest molecules absorbed.
  • Sorption of different molecules causes changes in symmetry and structure, which is vital for optimizing MOFs for technologies that rely on sorption.
View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!