Antimutagenic components in Dunn against -methyl--nitrosourea.

Genes Environ

Faculty of Pharmaceutical Sciences, Sanyo-onoda City University, Daigakudo-ri 1-1-1, Sanyo-onoda-shi, Yamaguchi, 756-0884 Japan.

Published: December 2019

Background: An extract from () Dunn has been reported to show potent antimutagenic effects against -alkyl--nitrosoureas in screening. The aim of this study was to identify the antimutagenic components from extracts of against -methyl--nitrosourea (MNU) in the Ames assay with strain TA1535 and to elucidate the antimutagenic mechanism of the flavonoids.

Results: From the ethyl acetate fraction obtained from fractionation of the methanol extract of Dunn, medicarpin, formononetin and isoliquiritigenin were successfully isolated through a combination of normal- and reversed-phase chromatography. Genistein and naringenin, which were already reported to be contained in Dunn, were also tested for their antimutagenicity towards MNU, along with formononetin, isoliquiritigenin and medicarpin. Our results demonstrated that genistein, isoliquiritigenin, medicarpin and naringenin were antimutagenic against MNU without showing cytotoxicity. MNU is reported to cause not only DNA alkylation but also induce reactive oxygen species. The hydroxyl radical scavenging capacity of the flavonoids was correlated with the antimutagenic capacity, indicating that the hydroxyl radical scavenging activity was involved in their antimutagenicity towards MNU.

Conclusions: It is important to prevent DNA damage by -nitrosamines for cancer chemoprevention. Genistein, isoliquiritigenin, medicarpin and naringenin were demonstrated to possess an antigenotoxic effects against carcinogenic MNU due to their radical scavenging activity.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6907206PMC
http://dx.doi.org/10.1186/s41021-019-0137-4DOI Listing

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