In order to study the construction of digital three-dimensional model of rabbit vascular network and provide a powerful basis for the model construction and image processing of human vascular network, in this study, rabbit abdominal and pelvic vessels are perfused with latex-bismuth oxide contrast agent. After that, the internal fibrous structure of the abdominal and pelvic vascular network in rabbits is studied by micro-computed tomography (Micro-CT). Angiography and post-processing software are performed. Firstly, six female rabbits are selected as the study subjects and are anesthetized by intraperitoneal injection of 12% chloral hydrate. After complete anesthesia, laparotomy is performed on the rabbits. The abdominal wall of the rabbit is cut longitudinally to expose the pelvic and abdominal cavity completely. The posterior peritoneum is found and opened. The catheter is inserted into the abdominal aorta and renal artery to complete the abdominal aorta and arterial intubation. Latex, bismuth oxide and potassium hydroxide are mixed in a ratio of 1:1:1 to form latex-bismuth oxide mixed solution as a mixed contrast agent for blood vessel perfusion. After the rabbit is perfused with mixed contrast medium, four organs, including bladder, uterus, small intestine and kidney, are selected as the research organs to construct the three-dimensional model of vascular network in this study. Finally, the above organs are scanned in vitro by micro-CT technology, and the original images of each organ are processed. Then, Mimics 17.0 software is used to build a digital three-dimensional model of the abdominal and pelvic vascular network in rabbits, obtain the information parameters of each network, and analyze the classification and diameter of blood vessels. The results show that the classification of bladder vascular network can only be divided into two levels, uterine vascular network can be divided into three levels, and small intestine and kidney vascular network can be divided into four levels. Therefore, the combination of micro-CT scanning and contrast agent can successfully construct a digital three-dimensional model of rabbit vascular network, which provides a new idea and method for the study of human abdominal and pelvic organs and physiological characteristics.
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http://dx.doi.org/10.1016/j.sjbs.2019.09.026 | DOI Listing |
Mol Ther
January 2025
Department of Molecular Medicine, University of Southern Denmark; Odense, 5230, Denmark. Electronic address:
Neovascular age-related macular degeneration and diabetic macular edema are leading causes of vision-loss evoked by retinal neovascularization and vascular leakage. The glycoprotein microfibrillar-associated protein 4 (MFAP4) is an integrin αβ ligand present in the extracellular matrix. Single-cell transcriptomics reveal MFAP4 expression in cell-types in close proximity to vascular endothelial cells including choroidal vascular mural cells and retinal astrocytes and Müller cells.
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Department of Vascular Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, China; Metabolic Vascular Disease Key Laboratory of Sichuan Province, The Affiliated Hospital of Southwest Medical University, 646000 Luzhou, China; Key Laboratory of Medical Electrophysiology, Ministry of Education & Medical Electrophysiological Key Laboratory of Sichuan Province, (Collaborative Innovation Center for Prevention of Cardiovascular Diseases) Institute of Cardiovascular Research, Southwest Medical University, Luzhou, 646000, China; Department of General Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, China. Electronic address:
Vinculin, a crucial adhesion plaque protein, plays a significant role in cell morphology and tissue development. Dysregulation of focal adhesion proteins has been linked to numerous diseases, including cardiovascular conditions, gastrointestinal disorders, and cancer. Recent studies increasingly highlight vinculin's involvement in the progression of these diseases; however, a comprehensive review remains lacking.
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College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China; Co-Construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan & Education Ministry of P.R. China, Henan University of Chinese Medicine, Zhengzhou 450046, China; Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Medicine, Zhengzhou 450046, China. Electronic address:
Tripterygium wilfordii (TW), which has severe hepatotoxicity, is commonly used as anti-rheumatism. Using the juice of auxiliary herbs in concocting poisonous herbs is a conventional method for toxicity reduction or efficacy enhancement. Traditional Chinese Pharmacy textbooks record that Spatholobi Caulis (SC) can alleviate the side effects caused by TW and also possesses excellent hepatoprotective effect.
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Cardiovascular Department, Fondazione Poliambulanza, 25124 Brescia, Italy.
This study assessed the proportion of secondary cardiovascular prevention patients who achieved low-density lipoprotein (LDL) cholesterol targets as per the 2019 ESC/EAS Dyslipidemia Guidelines. We also evaluated whether lipid-lowering therapies (LLTs) were adjusted in patients not meeting targets and analyzed the likelihood of these modifications achieving recommended levels. A multicenter, cross-sectional observational study retrospectively reviewed medical records of 1909 outpatients in 9 Italian cardiac rehabilitation/secondary prevention clinics from January 2023 to June 2024.
View Article and Find Full Text PDFMolecules
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Grupo Biomateriales Dentales, Escuela de Odontología, Universidad del Valle, Calle 4B # 36-00, Cali 760001, Colombia.
Scaffolds for regenerative therapy can be made from natural or synthetic polymers, each offering distinct benefits. Natural biopolymers like chitosan (CS) are biocompatible and biodegradable, supporting cell interactions, but lack mechanical strength. Synthetic polymers like polyvinyl alcohol (PVA) provide superior mechanical strength and cost efficiency but are not biodegradable or supportive of cell adhesion.
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