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Enhancing Neurogenesis of Neural Stem Cells Using Homogeneous Nanohole Pattern-Modified Conductive Platform. | LitMetric

AI Article Synopsis

  • Researchers developed biocompatible platforms with specific nanohole patterns that help control how stem cells grow and differentiate.
  • These platforms use laser interference lithography (LIL) to easily adjust the size of nanoholes, which impact cell adhesion and spreading.
  • The 500 nm-sized nanoholes were found to be most effective for promoting neurogenesis in mouse neural stem cells, suggesting that this technology could improve how stem cells are cultured and guided toward different functions.

Article Abstract

Biocompatible platforms, wherein cells attach and grow, are important for controlling cytoskeletal dynamics and steering stem cell functions, including differentiation. Among various components, membrane integrins play a key role in focal adhesion of cells (18-20 nm in size) and are, thus, highly sensitive to the nanotopographical features of underlying substrates. Hence, it is necessary to develop a platform/technique that can provide high flexibility in controlling nanostructure sizes. We report a platform modified with homogeneous nanohole patterns, effective in guiding neurogenesis of mouse neural stem cells (mNSCs). Sizes of nanoholes were easily generated and varied using laser interference lithography (LIL), by changing the incident angles of light interference on substrates. Among three different nanohole patterns fabricated on conductive transparent electrodes, 500 nm-sized nanoholes showed the best performance for cell adhesion and spreading, based on F-actin and lamellipodia/filopodia expression. Enhanced biocompatibility and cell adhesion of these nanohole patterns ultimately resulted in the enhanced neurogenesis of mNSCs, based on the mRNAs expression level of the mNSCs marker and several neuronal markers. Therefore, platforms modified with homogeneous nanohole patterns fabricated by LIL are promising for the precise tuning of nanostructures in tissue culture platforms and useful for controlling various differentiation lineages of stem cells.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981825PMC
http://dx.doi.org/10.3390/ijms21010191DOI Listing

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