AI Article Synopsis
- A 3D culture system mimicking the natural environment of bone is important for creating bone-like material in labs.
- This study uses plant-derived cellulose scaffolds to support the growth of stem cells turning into osteoblasts (bone cells).
- Among the tested materials, apple scaffolds with 300 μm pores performed the best, leading to successful tissue generation that could be implanted in rats, promoting bone formation.
Article Abstract
A three-dimensional (3D) culture system that closely replicates the in vivo microenvironment of calcifying osteoid is essential for in vitro cultivation of bone-like material. In this regard, the 3D cellulose constructs of plants may well serve as scaffolds to promote growth and differentiation of osteoblasts in culture. Our aim in this study was to generate bone-like tissue by seeding pluripotent stem cells (hiPSCs), stimulated to differentiate as osteoblasts in culture, onto the decellularised scaffolds of various plants. We then assessed expression levels of pertinent cellular markers and degrees of calcium-specific staining to gauge technical success. Apple scaffolding bearing regular pores of 300 μm seemed to provide the best construct. The bone-like tissue thus generated was implantable in a rat calvarial defect model where if helped form calcified tissue. Depending on the regularity and sizing of scaffold pores, this approach readily facilitates production of mineralized bone.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934596 | PMC |
| http://dx.doi.org/10.1038/s41598-019-56651-0 | DOI Listing |
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