Phase unwrapping is a necessary step in fringe-projection profilometry that produces accurate depth maps. However, the original wrapped phase is often corrupted by errors, and thus conventional spatial unwrapping suffers from error propagation, such as scanline-based unwrapping, and high complexity, such as quality-guided methods. In this paper, we propose a fast and robust spatial unwrapping method called multi-anchor scanline unwrapping (MASU). Different from previous work, when unwrapping each pixel, MASU refers to multiple anchors in the scanline, where each anchor has a threshold adapting to its location. In such a manner, a set of fringe order candidates are predicted by the anchors according to phase smoothness assumption, and the one with the highest number of votes is chosen. After that, with the obtained fringe order, the absolute phase and depth are computed. Simulation and experiments have shown that even corrupted by severe phase errors, the proposed MASU can still produce robust unwrapped results. In addition, MASU is thousands of times faster than quality-guided unwrapping with comparative or even superior depth accuracy.
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http://dx.doi.org/10.1364/OE.27.033488 | DOI Listing |
Sci Rep
January 2025
Molecular Modeling and Simulation Team, Institute for Quantum Life Science, National Institutes for Quantum Science and Technology, 4-9-1, Anagawa, Inage-Ku, Chiba City, Chiba, 263-8555, Japan.
Sequence-dependent mechanical properties of DNA could play essential roles in nuclear processes by affecting histone-DNA interactions. Previously, we found that the DNA entry site of the first nucleosomes from the transcription start site (+ 1 nucleosome) in budding yeast enriches AA/TT steps, but not the exit site, and the biased presence of AA/TT in the entry site was associated with the transcription levels of yeast genes. Because AA/TT is a rigid dinucleotide step, we considered that AA/TT causes DNA unwrapping.
View Article and Find Full Text PDFWe present a non-interferometric technique for quantitative phase imaging (QPI) that is cost-effective, easily integrated into standard microscopes, and capable of wide-field imaging with noncoherent light. Our method measures the phase gradient through optical differentiation using spatially variable amplitude filters, accommodating a range of transmission functions, including commercially available variable neutral-density filters. This flexibility is made possible by a general relationship we derive.
View Article and Find Full Text PDFSensors (Basel)
December 2024
College of Civil Engineering, Xiangtan University, Xiangtan 411105, China.
Bridge expansion joints are critical components that accommodate the movement of a bridge caused by temperature fluctuations, concrete shrinkage, and vehicular loads. Analyzing the spatiotemporal deformation of these expansion joints is essential for monitoring bridge safety. This study investigates the deformation characteristics of Hongtang Bridge in Fuzhou, China, using synthetic aperture radar interferometry (InSAR).
View Article and Find Full Text PDFSensors (Basel)
December 2024
Smart Computational Imaging Laboratory (SCILab), School of Electronic and Optical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
Lens-free on-chip microscopy (LFOCM) is a powerful computational imaging technology that combines high-throughput capabilities with cost efficiency. However, in LFOCM, the phase recovered by iterative phase retrieval techniques is generally wrapped into the range of -π to π, necessitating phase unwrapping to recover absolute phase distributions. Moreover, this unwrapping process is prone to errors, particularly in areas with large phase gradients or low spatial sampling, due to the absence of reliable initial guesses.
View Article and Find Full Text PDFJ Chem Phys
January 2025
Department of Physics and Astronomy and Center for Quantitative Biology, Rutgers University, Piscataway, New Jersey 08854, USA.
Nucleosomes are fundamental units of chromatin in which a length of genomic DNA is wrapped around a histone octamer spool in a left-handed superhelix. Large-scale nucleosome maps show a wide distribution of DNA wrapping lengths, which in some cases are tens of base pairs (bp) shorter than the 147 bp canonical wrapping length observed in nucleosome crystal structures. Here, we develop a thermodynamic model that assumes a constant free energy cost of unwrapping a nucleosomal bp.
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