AI Article Synopsis
Article Abstract
MicroRNAs (miRNAs) have recently received a significant amount of attention due to their remarkable influence on post-transcriptional gene regulation. In this study, we aim to provide a catalogue of miRNAs present in spermatozoa, seminal plasma and testicular tissue. Expression profiles of miRNA in spermatozoa and seminal plasma of 16 proven fertile men and testicular tissue of eight men with morphologically and/or histologically confirmed obstructive azoospermia were determined by microarray and RT-qPCR in combination with bioinformatics analyses. A total of 123, 156 and 133 miRNAs were consistently detected in spermatozoa, seminal plasma and testicular tissue respectively. Sixty-four miRNAs were shared across all sample types. Based on miRNAs expression level present in each group, correlation analysis showed moderate-to-strong correlations within the spermatozoa and seminal plasma samples and a wider range of correlations within the testicular tissue samples. The target genes of known miRNAs appeared to be involved in a wide range of biological processes related to reproduction, development and differentiation of germ cells. Our results suggest that there is a certain similarity between spermatozoa and seminal plasma for the relative miRNA expression changes with respect to testicular tissue and provide an overview of the miRNAs present in each sample type.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/and.13503 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Understanding the Crucial Role of Seminal Plasma Exosomes in Bull Fertility: A Review.
Reprod Domest Anim
December 2024
Animal Reproduction, Gynaecology and Obstetrics, Artificial Breeding Research Centre (ABRC), ICAR-National Dairy Research Institute, Karnal, Haryana, India.
Bull fertility is a multi-factorial trait and is affected by many factors, such as nutrition, genetics, and epigenetics. Superior quality male germplasm with high genetic merit helps to improve the livestock production trait. To achieve the target of livestock production, the availability of superior male germplasm is a great concern.
View Article and Find Full Text PDFComparison between a new device for the semen quality analysis and the manual microscopic evaluation in a not specialistic clinical laboratory.
Adv Lab Med
December 2024
Clinical Biochemistry Laboratory, Provincial Hospital of Bolzano (SABES-ASDAA), Bolzano, Italy.
Objectives: Semen analysis investigates different parameters of human semen with a high relevance in fertility workup, confirmation of sterility by post vasectomy, in pathologies follow-up such as varicocele and in all cases where sperm preservation is required. Manually seminal fluid examination is characterized by poor reproducibility. Aim of this study was to evaluate the performance of an automatic device in semen analysis by comparing its results with those obtained with the manual microscopy.
View Article and Find Full Text PDF[Effects of different fractions of Polygonati Rhizoma on reproductive dysfunction in male mice with kidney essence deficiency].
Zhongguo Zhong Yao Za Zhi
October 2024
School of Pharmaceutical Sciences, Zhejiang Chinese Medical University Hangzhou 310053, China.
This study aims to reveal the effects and mechanisms of different fractions of Polygonati Rhizoma on the reproductive dysfunction in male mice with kidney essence deficiency due to excess of sexual intercourse. Fifty male ICR mice with good sexual function were selected and randomized into normal(NC), model(MC), n-butanol fraction of Polygonati Rhizoma(0.4 g·kg~(-1), HJCT), remaining fraction of Polygonati Rhizoma(0.
View Article and Find Full Text PDFProteomics and Metabolomics in Varicocele-Associated Male Infertility: Advancing Precision Diagnostics and Therapy.
J Clin Med
December 2024
Third Department of Urology, Attikon University Hospital, School of Medicine, National and Kapodistrian University of Athens, 12462 Athens, Greece.
Varicoceles are a common contributor to male infertility, significantly impacting male-factor infertility cases. Traditional diagnostic methods often lack the sensitivity to detect the molecular and cellular disruptions caused by varicoceles, limiting the development of effective, personalized treatments. This narrative review aims to explore the advancements in proteomics and metabolomics as innovative, non-invasive diagnostic tools for varicocele-associated male infertility and their potential in guiding personalized therapeutic strategies.
View Article and Find Full Text PDFSustainable Alternatives to the Reduction of Plastic Straws Used with Chilled Equine Semen.
Animals (Basel)
November 2024
Department of Animal Pathology, Faculty of Veterinary Sciences, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain.
Currently, plastic is used being without any limitations. The livestock sector is no stranger to its use. For example, artificial insemination involves the use of different plastic materials, including semen straws and insemination catheters, which increase the levels of waste in our environment.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!