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[Preparation of angiopep-2-modified FITC-labeled neurotoxin nanoparticles and in vitro release characteristics]. | LitMetric

AI Article Synopsis

  • Researchers used an emulsion/solvent evaporation method to create angiopep-2 modified neurotoxin nanoparticles (ANG-NPs/FITCNT) with m PEG-PLA and ANG-PEG-PLA as carriers and FITC-NT as the drug.
  • They found that optimizing ultrasound power (90 W) and time (30 s) resulted in nanoparticles with an average size of 123.9 nm, a zeta potential of -10.5 mV, and a high encapsulation efficiency of 68.1%.
  • The in vitro release of the drug from these nanoparticles in PBS buffer (at pH 7.4 and pH 6.5) followed a predictable pattern, indicating that these nanoparticles

Article Abstract

In order to prepare angiopep-2 modified fluorescein isothiocyanate-labeled neurotoxin nanoparticles( ANG-NPs/FITCNT),emulsion/solvent evaporation method was used with m PEG-PLA and ANG-PEG-PLA( in proper proportions) as carriers and with FITC-NT as drug. With particle size and encapsulation efficiency as comprehensive indexes,the effects of different ultrasound power and ultrasound time combinations on the process were investigated. The in vitro release characteristics of nanoparticles in PBS buffer at p H 7. 4 and p H 6. 5 were investigated by dialysis method. The results indicated that the optimum process for preparing ANG-NPs/FITC-NT was as follows: ultrasonic power 90 W,ultrasonic time 30 s. In such optimal process,ANG-NPs/FITC-NT were well-shaped under the transmission electron microscope,with an average particle size of( 123. 9±0. 5) nm,Zeta potential of(-10. 5±0. 5) m V,encapsulation efficiency of( 68. 1±0. 4) %,and the drug loading of( 0. 82±0. 01) %. The in vitro drug release profiles of the nanoparticles in PBS buffer at p H 7. 4 and p H 6. 5 were both consistent with Ritger-Peppas equation,ln Q = 0. 508 8 lnt-2. 285 0,r = 0. 961 5( p H 7. 4) and ln Q= 0. 449 9 lnt-1. 855 3,r = 0. 970 3( p H 6. 5),respectively. The experiment results proved that the nanoparticles prepared by emulsion/solvent evaporation method had uniform particle size,high encapsulation efficiency and in vitro sustained release characteristic,which might be a potential carrier for NT intracerebral drug delivery.

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Source
http://dx.doi.org/10.19540/j.cnki.cjcmm.20190630.301DOI Listing

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