Presegmentation Procedure Generates Smooth-Sided Microfluidic Devices: Unlocking Multiangle Imaging for Everyone?

ACS Omega

Division of Infection Medicine, Department of Clinical Sciences, Lund University, Lund 221 84, Sweden.

Published: December 2019

We present a simple procedure to create smooth-sided, transparent polymer-based microfluidic devices by presegmentation with hydrophobized glass slides. We study the hypothesis that the smooth side planes permit rapid multiangle imaging of microfluidic systems in contrast to the turbid side planes that result from cutting the polymer. We compare the compatibility of the entire approach with the conventional widefield microscopy, confocal and 2-photon microscopy, as well as three-dimensional (3D) rendering and discuss limitations and potential applications.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6921255PMC
http://dx.doi.org/10.1021/acsomega.9b02139DOI Listing

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