This study aims to determine the antioxidant activity of bioactive peptides derived from sp. VDW cells cultured for 21 days. sp. VDW protein hydrolysates were prepared with trypsin and purified by ultrafiltration with molecular mass cut-off membranes of 10, 5 and 3 kDa. The <3 kDa (F) fraction had the highest 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, with IC values of (11.5±0.3) and (13.6±0.2) µg/mL, respectively. The F fraction was separated by reversed phase HPLC to yield four subfractions (F). The F subfraction showed the highest maximum ABTS radical scavenging activity (3.55±0.61) % and it was selected for further analysis by electrospray ionisation quadrupole time-of-flight mass spectrometry (ESI-Q-TOF-MS/MS) based on peptide sequencing. Five antioxidant peptides were identified, of which AILESYSAGKTK had the highest ABTS radical scavenging activity. Furthermore, the F fraction showed high cytotoxic activities against human cancer-derived cell lines, especially the colon cancer cell line (SW620) with an IC value of (106.6±21.5) µg/mL, but not the untransformed Wi38 cell line. The F fraction activated the apoptotic pathway in SW620 cells after treatment for 24, 48 and 72 h, with the highest activities of caspases-3, -8 and -9 being observed after treatment for 72 h. These findings suggested that microalgae sp. VDW may be used to develop natural anticancer drugs.
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| http://dx.doi.org/10.17113/ftb.57.03.19.6028 | DOI Listing |
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