AI Article Synopsis
- A new enzymatic bioassay strategy using gold nanoclusters (AuNCs) is introduced, which relies on their photoluminescence being quenched by ferricyanide but not by ferrocyanide, making it a useful fluorescent sensing platform.
- The method utilizes enzymes like glucose oxidase and a lactate dehydrogenase/diaphorase cascade to convert ferricyanide to ferrocyanide, which activates the fluorescence, allowing sensitive measurement of glucose and lactate in small concentrations (0.12 and 0.09 μM, respectively).
- This approach is the first to leverage the ferricyanide/ferrocyanide redox couple in fluorescent bioanalysis, providing enhanced sensitivity and select
Article Abstract
Herein we present a general and turn-on strategy for enzymatic bioassays on the basis of redox state dependent emission of gold nanoclusters (AuNCs). The photoluminescence of AuNCs was quenched obviously by the oxidative ferricyanide while unaffected by its corresponding reduced state, i.e., ferrocyanide. The distinctive quenching abilities for AuNCs by the redox couple (ferricyanide/ferrocyanide) enabled their utility as new fluorescent sensing platforms to detect redox-related phenomena. The proposed protocols were conducted by using the model oxidoreductases of glucose oxidase (GOx) and the enzyme cascade of lactate dehydrogenase (LDH)/diaphorase to catalytically convert ferricyanide to ferrocyanide, which switched on fluorescence of the detection systems. The detection limit for glucose and lactate was found to be as low as 0.12 and 0.09 μM, respectively. This work features the first use of the redox couple of ferricyanide/ferrocyanide in fluorescent bioanalysis, which enables versatile, signal on and highly sensitive/selective detections as compared to the state of the art fluorescently enzymatic sensing platforms. Importantly, considering the significance of ferricyanide/ferrocyanide involves in numerous other oxidoreductases mediated biocatalysis, this protocol has wide versatility that enables combination with oxidoreductases related reactions for biosensing.
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| http://dx.doi.org/10.1016/j.aca.2019.10.035 | DOI Listing |
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