Photo-CIDNP is one among different promising techniques to improve nuclear magnetic resonance (NMR) sensitivity. Being sensitive to particular amino acids makes it a promising candidate with respect to biological NMR. Our goal is to exploit new sensitizers and compare them to the present working horses flavin mononucleotide, bipyridyl, and the recently applied fluorescein. Among the investigated dyes, we found a new and very efficient one, Atto Thio 12, conveniently used in fluorescence microscopy with 2-3 fold enhanced polarization when compared to the established ones. We also show that diode lasers which are now available from near UV to IR can be efficiently used in photo-CIDNP. Results are obtained with the amino acids tyrosine and tryptophan.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1063/1.5128575 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Molecular features toward high photo-CIDNP hyperpolariztion explored through the oxidocyclization of tryptophan.
Phys Chem Chem Phys
March 2021
Laboratory of Physical Chemistry, Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland.
Article Synopsis
- Photo-CIDNP (photo-chemically induced dynamic nuclear polarization) enhances NMR spectroscopy sensitivity, particularly in biological systems, but traditionally offers lower signal enhancement compared to other hyperpolarization methods like DNP and SABRE.
- A new photo-oxidation product of tryptophan, identified as 3α-hydroxypyrroloindole, shows significant signal enhancements of up to 380-fold for hydrogen at 200 MHz, leading to measurement time reductions of up to 144,400-fold.
- The research reveals that this oxidation product is highly effective under continuous wave light irradiation, providing insights into mechanisms behind its improved signal performance over regular tryptophan.
Atto Thio 12 as a promising dye for photo-CIDNP.
J Chem Phys
December 2019
Laboratory of Physical Chemistry, Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland.
Photo-CIDNP is one among different promising techniques to improve nuclear magnetic resonance (NMR) sensitivity. Being sensitive to particular amino acids makes it a promising candidate with respect to biological NMR. Our goal is to exploit new sensitizers and compare them to the present working horses flavin mononucleotide, bipyridyl, and the recently applied fluorescein.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!