() associated infections are one of the major threats to public health. The aim of the present study was to determine the antibiotic resistance pattern as well as the genetic characterization of methicillin and vancomycin resistant (VRSA) isolated from a tertiary care hospital in Lahore. The were isolated from different clinical samples, identified by biochemical testing, and subjected to antibiotic susceptibility testing by disc diffusion method or broth microdilution method. Methicillin resistance gene (A) and vancomycin resistance gene (A) were amplified by polymerase chain reaction. The isolates showed high incidence of resistance against methicillin (76%) and moderate incidence of resistance to vancomycin (44%). Isolates were also resistant to several other drugs such as cefoxitin (76%), ertapenem (83%), ampicillin (81%), tobramycin (78%), moxifloxacin (76%), and tetracycline (74%). An encouraging finding was that 98% of isolates were susceptible to tigecycline, indicating its possible role in the treatment of MRSA, VRSA, as well as multi-drug resistant . The A gene was detected from 33.3% (10/30) while A gene was detected from 46.6% (14/30) of the tested isolates. In conclusion, frequent presence of methicillin and vancomycin resistance in appraises the cautious use of these antibiotics in clinical practices. Furthermore, it is suggested that there should be continuous monitoring of tigecycline treatments in clinical setups in order to delay the development of resistance against it.
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http://dx.doi.org/10.3390/antibiotics9010003 | DOI Listing |
Front Microbiol
January 2025
Doctoral Program of Biosciences, School of Engineering, Universidad de La Sabana, Chía, Cundinamarca, Colombia.
Marine-derived actinobacteria isolated from sponge and soft coral were screened for antibacterial activity against acne-related bacteria, specifically ATCC 14990, methicillin-resistant ATCC BAA44, and ATCC 6919. Cytotoxicity assays were performed on human dermal fibroblast (HDFa) and keratinocyte (HaCaT) cell lines to assess the safety profile of the extracts. Chemical characterization was conducted using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS).
View Article and Find Full Text PDFFood Chem Toxicol
January 2025
Department of Biochemistry, Faculty of Pharmacy, Mansoura University, 35516 Mansoura, Egypt.
Vancomycin, a glycopeptide antibiotic, is used in cases of drug-resistant bacterial infections, but unfortunately is associated with acute kidney injury (AKI). We here explore the protective potential of aprepitant against vancomycin-induced AKI. Vancomycin (500 mg/kg/i.
View Article and Find Full Text PDFJ Infect Dev Ctries
December 2024
Ankara Etlik City Hospital, Department of Medical Microbiology, Ankara, Turkey.
Introduction: Antimicrobial resistance remains a global threat with increasing morbidity and mortality rates. The aim of this study was to identify the antimicrobial resistance trends among ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) isolated from clinical samples at a Health Practice and Research Hospital over five years.
View Article and Find Full Text PDFJ Infect Dev Ctries
December 2024
Department of Microbiology & Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Introduction: The emergence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is a growing public health concern. The objective of this study was to determine the prevalence and multi-drug resistant (MDR) profiles of MRSA in goats in Bangladesh.
Methodology: A total of 150 samples from goats comprised of rectal swab (n = 50), nasal swab (n = 50), and milk (n = 50) were collected.
J Pharm Policy Pract
January 2025
Shifa College of Pharmaceutical Sciences, Shifa Tameer e Milat University, Pakistan.
Background: Antibiotics are widely used medications among infectious disease patients; therefore, proper monitoring and assessment are critical for ensuring rational use. Antimicrobial stewardship addresses the rational and appropriate use of antibiotics, which reinforces overall health outcomes. Ongoing antimicrobial resistance scenarios are an alarming condition for healthcare, necessitating continued practice of such assessments.
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