Bioactive Secondary Metabolites from the Culture of the Mangrove-Derived Fungus HJ004.

Two new polyketides, 8--methylnodulisporin F () and nodulisporin H (), two new naphthoquinones, 5-hydroxy-2-methoxy-6,7-dimethyl-1,4-naphthoquinone () and 5-hydroxy-2-methoxynaphtho[9-]furan-1,4-dione (), and a new naphthofuran 1,3,8-trimethoxynaphtho[9-]furan (), along with five known compounds 4--methyl eleutherol (), 2-acetyl-7-methoxybenzofuran (), (-)-orthosporin (), diaporthin (), and 6-hydroxymellein (), were obtained from the EtOAc extract of the mangrove-derived fungus HJ004. The structures of the isolated compounds were elucidated by extensive NMR and MS analyses, while the absolute configurations of the stereogenic carbons were established based on experimental and calculated electronic circular dichroism spectra. Compounds and displayed a potent inhibitory activity against -glucosidase with the IC values of 5.7 and 1.1 μg/mL, respectively. Compounds and showed a moderate antibacterial activity against , methicillin-resistant (MRSA) and , with minimum inhibitory concentration (MIC) values ranging from 6.25 to 12.5 μg/mL. Compound exhibited antibacterial activity against with the MIC value of 12.5 μg/mL.