AI Article Synopsis
- We introduce a method for creating customizable multiassay platforms using 3D printing of PEGDA hydrogels, which have low protein-binding properties ideal for muscle tissue analysis.
- The method involves immobilizing cells in a hydrogel matrix with embedded cantilevers, essential for forming robust muscle tissues.
- By controlling the 3D shape and mechanical properties during printing, we can optimize tissue strength and reduce stress, mitigating the risk of tissue fractures.
Article Abstract
We present a method for reproducible manufacture of multiassay platforms with tunable mechanical properties for muscle tissue strip analysis. The platforms result from stereolithographic 3D printing of low protein-binding poly(ethylene glycol) diacrylate (PEGDA) hydrogels. Contractile microtissues have previously been engineered by immobilizing suspended cells in a confined hydrogel matrix with embedded anchoring cantilevers to facilitate muscle tissue strip formation. The 3D shape and mechanical properties of the confinement and the embedded cantilevers are critical for the tissue robustness. High-resolution 3D printing of PEGDA hydrogels offers full design freedom to engineer cantilever stiffness, while minimizing unwanted cell attachment. We demonstrate the applicability by generating suspended muscle tissue strips from C2C12 mouse myoblasts in a compliant fibrin-based hydrogel matrix. The full design freedom allows for new platform geometries that reduce local stress in the matrix and tissue, thus, reducing the risk of tissue fracture.
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| http://dx.doi.org/10.1021/acs.biomac.9b01274 | DOI Listing |
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