Colocalization of GABA and [Met]enkephalin-Arg6-Gly7-Leu8 in the rat cerebellum.

The distribution of [Met]enkephalin-Arg6-Gly7-Leu8-like immunoreactivity (MEAGL-LI) in the rat cerebellum was investigated by peroxidase anti-peroxidase immunocytochemistry using specific antiserum against MEAGL. MEAGL-LI positive neuronal perikarya were distributed in the granular layer, and they seemed to correspond to Golgi cells from their size and location. In addition, diffusely and weakly stained neuronal perikarya were also observed in the molecular layer. Immunoreactive fibers and terminals were found in the granular layer. Furthermore, examination of serial frozen sections (4-6 micron in thickness) from rats pretreated with colchicine clarified the colocalization of gamma-aminobutyric acid (GABA) and MEAGL in Golgi cells but not in the stellate cells.