[Cloning and expression analysis of chicken circular transcript of insulin degrading enzyme gene].

Insulin-degrading enzyme (IDE) is a highly conserved metallopeptidase that functions in the catabolism of bioactive peptides. In our previous study, we identified a putative circular transcript in that chicken insulin-degrading enzyme () gene through analyzing a high throughput sequencing result. Here we set to confirm the circular transcript of () and explore its expression regularity in normal barred Plymouth chicken. The was confirmed by PCR amplification and sequencing. The circular structure of was determined by RNase R processing and reverse transcription experiments. Then we analyzed the spatiotemporal expression pattern of and mRNA and compared the differential expression of and mRNA in the normal barred Plymouth chicken and the dwarf ones. The results showed that the full length of chicken was 1332 nt, divided form exon 2-11 of the gene. RNase R tolerance analysis showed that chicken had the general characteristics of circular molecule, and was highly resistant to RNase R. The random primers had higher transcription efficiency than the oligo-d(T) primers, confirming that is a circular structured molecule without poly(A). was highly expressed in the liver and heart tissues but less in the muscle tissues of leg and breast in normal chickens at the age of 1 and 12 weeks. The expression profile of in liver tissue showed that level was lower in1 to 6 weeks and then became higher after 8 weeks of age. The expression of in liver tissue was significantly higher in normal chicken than that in dwarf barred Plymouth chicken (<0.05). This study confirmed a strucutre in chicken gene and uncovered its expression regularity. We demonstrated that the expression level of in the liver tissue was higher in normal barred Plymouth chicken compared to dwarf species. This study paves the way for further understanding the biological function of chicken including its roles in regulating chicken growth and development.