Antimicrobial Resistance and Molecular Characteristics of Methicillin-resistant Isolates from Children Patients in Iran.

Introduction: Methicillin-resistant  (MRSA) causes high rates of mortality and a substantial burden to health systems worldwide. Here, we investigated the antimicrobial susceptibility and molecular characteristics of MRSA isolated from children referred to Children's Medical Center in Tehran.

Materials And Methods: A total of 98 MRSA isolates were collected from children. Antimicrobial resistance patterns were determined using the disk diffusion and E-test methods. The presence of biofilm encoding genes and the gene were determined by PCR. We used the microtiter plate method to assess the ability of biofilm formation. The MRSA isolates were further analyzed using PFGE and SCCmec typing.

Results: Antibiotic susceptibility testing showed that the highest and the lowest antibiotic resistance percentage were related to erythromycin (62%) and minocycline (10%), respectively. Overall, 63% of MRSA isolates were biofilm producers. Resistance to two antibiotics such as erythromycin (72% vs 28%, =0.01) and clindamycin (71% vs 29%, =0.04) was higher among biofilm producers than non-biofilm producers. All strains had biofilm-forming genes and the prevalence of gene was 41%. Most MRSA isolates belonged to (75%) and (18%). In PFGE technique, 5 common types and 2 single types were identified; Common type 1 with 37 isolates was dominant clone.

Conclusion: We thus report preliminary data on the prevalence and distribution of MRSA genotypes in Tehran Children's Hospital. These findings characterize the MRSA colonization dynamics in child patients in Iran and may aid the design of strategies to prevent MRSA infection and dissemination.