AI Article Synopsis
- Routine therapeutic drug monitoring is crucial due to the risk of drug-drug interactions from common antidepressant combinations.
- A novel method using C-functionalized magnetic silica nanoparticles enables effective extraction of several antidepressants from plasma and urine, followed by ultra-HPLC-MS/MS analysis.
- The technique demonstrated high sensitivity, accuracy, and precision, making it suitable for analyzing clinical samples in therapeutic drug monitoring.
Article Abstract
Routine therapeutic drug monitoring is highly recommended since common antidepressant combinations increase the risk of drug-drug interactions or overlapping toxicity. A magnetic solid-phase extraction by using C-functionalized magnetic silica nanoparticles (C-FeO@SiO NPs) as sorbent was proposed for rapid extraction of venlafaxine, paroxetine, fluoxetine, norfluoxetine and sertraline from clinical plasma and urine samples followed by ultra-HPLC-MS/MS assay. The synthesized C-FeO@SiO NPs showed high magnetization and efficient extraction for the analytes. After cleanup by magnetic solid-phase extraction, no matrix effects were found in plasma and urine matrices. The analytes showed LODs among 0.15-0.75 ng ml, appropriate linearity (R ≥ 0.9990) from 2.5 to 1000 ng ml, acceptable accuracies 89.1-110.9% with precisions ≤11.0%. The protocol was successfully applied for the analysis of patients' plasma and urine samples. It shows high potential in routine therapeutic drug monitoring of clinical biological samples.
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| http://dx.doi.org/10.4155/bio-2019-0171 | DOI Listing |
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