Antidengue potential of leaf extracts of and (Rubiaceae) against dengue virus and its vector (Diptera: Culicidae).

The aim of the present study was to screen the anti-dengue potential of crude leaf extracts of two plants from and . For larvicidal assay, the acetone extract of both plants showed maximum effects, with the least LC and LC values ( (5.968 and 7.493 μg/ml) and (1.288 and 1.992 μg/ml)) and the same extract of both plants exhibited better pupicidal potency. The adulticidal activity of both plants (0-60 min interval periods) recorded best results in acetone extracts and the LC and LC values were recorded as (32.105 and 41.001 μg/ml) and (09.012 and 11.854 μg/ml). Among the two plants acetone leaf extract have good antiviral property against Dengue viral cell line. In addition, the phytochemical nature of the plant reveals the presence of saponins, flavonoids and alkaloids in all the tested extracts of both plants. GC-MS analysis revealed Hexanedioic acid, Bis(2-Ethylhexyl) Ester (22.54) and 2,6,10,14,18,22- Tetracosahexane, 2,6,10, 15, 19,15,19,23- Hexamethyl-(ALL-E)- (25.33) identified as two major phytoconstitutents in and Tetracontane (23.580) is a major compound identified from acetone extracts. The functional groups of chemical compounds (aromatis, alkanes, alkyls and carboxylic acids) from and were analyzed by FT-IR spectrum.