Non-specific immunity promotion in response to garlic extract supplemented diets in female Guppy (Poecilia reticulata).

In the present study, the effect of aqueous garlic (Allium sativum) extract on skin mucus immune parameters of Poecilia reticulata was evaluated. A total of 240 P. reticulate juveniles, weighing 0.013 ± 0.001 g, were randomly stocked in 12 experimental glass tanks at a density of 30 fish per tank. The fish were fed with diets supplemented with garlic extract at the concentrations of 0, 0.10, 0.15, and 0.20 ml kg of diet three times a day at a ratio of 2.5% of their body weight for 80 days. Results of the study showed that, lysozyme activity increased significantly in the skin mucus of garlic-treated Guppy (P < 0.05). Values of ACH50 significantly increased (P < 0.05) in fish by increasing concentration of garlic extract from 0 to 0.15 ml kg in diet. ACH50 also showed a significant declining trend (P < 0.05) by increasing concentration of garlic extract from 0.15 to 0.20 ml kg. Total Ig content was enhanced significantly in 0.15 and 0.20 ml kg treatments as well. ALP activity significantly increased in garlic-fed treatments compared to the control. Inclusion of garlic extract in diet was found to have no significant effect on final body weight and weight gain. Results revealed that, administration of 0.15 mL of garlic extract per kg feed is suggested to obtain optimal skin mucus immunity in P. reticulata.