Here, we demonstrate use of a Mg-dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage efficiency is improved by use of a tandem-repeat cleavage site. We further demonstrate that DNAzyme-released oligos function as primers in reverse transcription of cell-released mRNA.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c9cc07790a | DOI Listing |
Publication Analysis
Top Keywords
dnazyme-powered nucleic
4
nucleic acid
4
acid release
4
release solid
4
solid supports
4
supports demonstrate
4
demonstrate mg-dependent
4
mg-dependent site-specific
4
site-specific dna
4
dna enzyme
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!