AI Article Synopsis
- This study investigates the effects of deleting the rnc gene in Streptococcus mutans on its biofilm formation and acid production, alongside the antibacterial efficacy of a compound called dimethylaminohexadecyl methacrylate (DMAHDM).
- Results indicate that the rnc-deleted strain is significantly more susceptible to both DMAHDM and chlorhexidine (CHX), leading to reduced biofilm biomass and lower production of harmful byproducts like polysaccharides and lactic acid.
- The combination of rnc deletion with DMAHDM treatment shows the highest effectiveness in inhibiting biofilms, suggesting that this dual approach could be promising for controlling dental biofilms and preventing tooth decay for the first time
Article Abstract
Objectives: Antibacterial quaternary ammonium monomers (QAMs) are used in resins. The rnc gene in Streptococcus mutans (S. mutans) plays a key role in resisting antibiotics. The objectives of this study were to investigate for the first time: (1) the effects of rnc deletion on S. mutans biofilms and acid production; (2) the combined effects of rnc deletion with dimethylaminohexadecyl methacrylate (DMAHDM) on biofilm-inhibition efficacy.
Methods: Parent S. mutans strain UA159 (ATCC 700610) and the rnc-deleted S. mutans were used. Bacterial growth, minimum inhibitory concentration (MIC), and minimal bactericidal concentration (MBC) were measured to analyze the bacterial susceptibility of the parent and rnc-deleted S. mutans against DMAHDM, with the gold-standard chlorhexidine (CHX) as control. Biofilm biomass, polysaccharide and lactic acid production were measured.
Results: The drug-susceptibility of the rnc-deleted S. mutans to DMAHDM or CHX was 2-fold higher than parent S. mutans. The drug-susceptibility did not increase after 10 passages (p < 0.05). Deleting the rnc gene increased the biofilm susceptibility to DMAHDM or CHX by 2-fold. The rnc-deletion in S. mutans reduced biofilm biomass, polysaccharide and lactic acid production, even at no drugs. DMAHDM was nearly 40 % more potent than the gold-standard CHX. The combination of rnc deletion+DMAHDM treatment achieved the greatest reduction in biofilm biomass, polysaccharide synthesis, and lactic acid production.
Significance: Gene modification by deleting the rnc in S. mutans reduced the biofilm growth and acid production, and the rnc deletion+DMAHDM method showed the greatest biofilm-inhibition efficacy, for the first time. The dual strategy of antibacterial monomer+bacterial gene modification shows great potential to control biofilms and inhibit caries.
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| http://dx.doi.org/10.1016/j.dental.2019.12.001 | DOI Listing |
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