Background: is a primarily foodborne bacterial pathogen that is one of the causative agents of gastroenteritis. However, the prevalence of infection in pediatric patients with diarrheal disease is not clearly identified in the Iranian population. This study aimed to investigate the frequency of isolates found in infectious diarrhea samples of pediatric patients in an Iranian population.
Methods: A total of 173 infectious diarrhea samples collected from pediatric patients were used in this crosssectional study. Samples were collected from patients referred to the Children's Educational-Therapeutic Center affiliated with the Arak University of Medical Sciences in Arak, Iran from May-September 2015. To identify the presence of , the samples were directly inoculated into the Listeria Enrichment Broth Base through cold enrichment, then plated onto isolated exclusive Listeria Selective Agar Base. As an alternative method for identifying , Polymerase Chain Reaction (PCR) of the gene was used.
Results: Of the 173 infectious diarrhea samples, eight (4.6%) with were identified using exclusive culture media, while nine (5.2%) were identified using PCR. The majority of infections (seven cases (77.7%)) were observed in children under the age of five.
Conclusion: Our results show infections to have a low prevalence for causing diarrhea in children in the central region of Iran. This should be taken into consideration by pediatricians when treating intestinal diseases.
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Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
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