AI Article Synopsis
- Microsatellite markers were created to assess genetic diversity and relationships in ex situ collections of the Campanulaceae family.
- A total of 40 potential markers were tested on individuals from two species and evaluated for cross-species use across various genera in the Hawai'ian lobelioid group.
- The study concludes that these primers are effective for measuring genetic diversity and relatedness, and they are broadly applicable for population genetic research within the Hawai'ian lobelioid group.
Article Abstract
Premise: Microsatellite markers were developed to measure genetic diversity and relatedness of ex situ collections of (Campanulaceae).
Methods And Results: Potential microsatellite markers were identified from two sources; 28 were developed for and an additional 12 markers from a previously published study of . Primer pairs were tested on 30 individuals of and 24 individuals of to provide measures of genetic diversity and inbreeding. We assessed cross-species amplification in an additional 13 taxa that represented all six genera within the Hawai'ian lobelioid group to determine the broader applicability of the markers.
Conclusions: Results indicate that these primers will provide useful estimates of genetic diversity and relatedness of ex situ collections of both species. In addition, we have also demonstrated the widespread applicability of these markers for use in population genetic studies of several species within the Hawai'ian lobelioid group.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6858292 | PMC |
| http://dx.doi.org/10.1002/aps3.11303 | DOI Listing |
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Article Synopsis
- Microsatellite markers were created to assess genetic diversity and relationships in ex situ collections of the Campanulaceae family.
- A total of 40 potential markers were tested on individuals from two species and evaluated for cross-species use across various genera in the Hawai'ian lobelioid group.
- The study concludes that these primers are effective for measuring genetic diversity and relatedness, and they are broadly applicable for population genetic research within the Hawai'ian lobelioid group.
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