AI Article Synopsis
- VHHs, or nanobodies from camelids, offer advantages over traditional antibodies in immunochemistry, showcasing high stability and specificity.
- Researchers isolated nanobodies specifically targeting Salmonella enteritidis through biopanning and developed a sandwich-ELISA assay with a detection limit of 1.4 × 10 CFU/mL.
- The assay successfully detected S. enteritidis in milk samples, demonstrating the practical application of nanobodies in bioanalytical methods.
Article Abstract
The variable domain of heavy chain only antibody (VHH), also named as nanobody obtained from camelid antibody libraries, has shown great potential in immunochemistry, with many advantages over conventional antibodies. Here, nanobodies towards Salmonella enteritidis (S. enteritidis) were isolated for the first time after biopanning of an immune camelid nanobody library. Those nanobodies showed high thermostability and good specificity. A sandwich-ELISA was developed with nanobody Nb 13, which exhibited a detection limit of 1.4 × 10 CFU/mL. The proposed assay was also applied to detect S. enteritidis in milk samples, which could detect 6 CFU/mL of S. enteritidis in milk after 10 h of enrichment. This study demonstrated an alternative strategy for S. enteritidis determination, and further proved the utility and practicality of nanobody-based reagents in bioanalytical chemistry.
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| http://dx.doi.org/10.1016/j.foodchem.2019.125942 | DOI Listing |
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