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Labeling of Proteins by BODIPY-Quinone Methides Utilizing Anti-Kasha Photochemistry. | LitMetric

Labeling of Proteins by BODIPY-Quinone Methides Utilizing Anti-Kasha Photochemistry.

ACS Appl Mater Interfaces

Department of Organic Chemistry and Biochemistry , Ruđer Bošković Institute, Bijenička cesta 54 , 10000 Zagreb , Croatia.

Published: January 2020

AI Article Synopsis

Article Abstract

A novel approach for the photolabeling of proteins by a BODIPY fluorophore is reported that is based on an anti-Kasha photochemical reaction from an upper singlet excited state () leading to the deamination of the BODIPY quinone methide precursor. On the other hand, the high photochemical stability of the dye upon excitation by visible light to allows for the selective fluorescence detection from the dye or dye-protein adduct, without concomitant bleaching or hydrolysis of the protein-dye adduct. Therefore, photolabeling and fluorescence monitoring can be uncoupled by using different excitation wavelengths. Combined theoretical and experimental studies by preparative irradiations, fluorescence, and laser flash photolysis fully disclose the photophysical properties of the dye and its anti-Kasha photochemical reactivity. The application of the dye was demonstrated on photolabeling of bovine serum albumin.

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Source
http://dx.doi.org/10.1021/acsami.9b19472DOI Listing

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