AI Article Synopsis
- * The study developed a dual sensing platform using graphene oxide (GO) for detecting miRNAs, incorporating a specially designed hairpin-like probe that includes a fluorescent dye and DNAzyme domain.
- * The new platform allows for observable color changes and enhances fluorescence sensitivity for miRNA detection at very low concentrations, all within a single solution without needing extra separation steps.
Article Abstract
MicroRNAs (miRNAs) are small non-coding RNAs, which are involved in RNA silencing and post-transcriptional regulation of gene expression. Numerous studies have determined the expression of certain miRNAs in specific tissues and cell types, and their aberrant expression is associated with a variety of serious diseases such as cancers, immune-related diseases, and many infectious diseases. This suggests that miRNAs may be attractive and promising non-invasive biomarkers of diseases. In this study, we established a graphene oxide (GO)-based fluorescence/colorimetric dual sensing platform for miRNA by using a newly designed probe. The probe was designed to form a hairpin-like configuration with a fluorescent dye-labeled long tail, possessing a guanine (G)-rich DNAzyme domain in the loop region and target binding domain over the stem region and tail. By introducing this new hairpin-like probe in a conventional GO-based fluorescence platform, we observed both the miRNA-responsive color change by direct observation and sensitive fluorescence increase even below the nanomolar levels in a single solution without an additional separation step.
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| http://dx.doi.org/10.1007/s00216-019-02269-0 | DOI Listing |
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