AI Article Synopsis
- The study developed a complex using human nasal septal cartilage (hNC) and polycaprolactone (PCL) for potential transplantation into cartilage defects.
- The research involved isolating hNC from patients and testing the cell-PCL complex for cell viability, growth, and protein expression relevant to cartilage health.
- Results indicated that the hNC-PCL complex maintained high viability and expressed important cartilage proteins, suggesting it could effectively repair cartilage injuries in clinical settings.
Article Abstract
Background: In this study, we manufactured a complex of human nasal septal cartilage (hNC) with polycaprolactone (PCL) for transplantation into cartilaginous skeletal defects and evaluated their characteristics.
Methods: Nasal septum tissue was obtained from five patients aged ≥ 20 years who were undergoing septoplasty. hNCs were isolated and subcultured for three passages . To formulate the cell-PCL complex, we used type I collagen as an adhesive between chondrocyte and PCL. Immunofluorescence staining, cell viability and growth in the hNC-PCL complex, and mycoplasma contamination were assessed.
Results: hNCs in PCL showed viability ≥ 70% and remained at these levels for 9 h of incubation at 4 °C. Immunostaining of the hNC-PCL complex also showed high expression levels of chondrocyte-specific protein, COL2A1, SOX9, and aggrecan during 24 h of clinically applicable conditions.
Conclusion: The hNC-PCL complex may be a valuable therapeutic agent for implantation into injured cartilage tissue, and can be used clinically to repair cartilaginous skeletal defects. From a clinical perspective, it is important to set the short duration of the implantation process to achieve effective functional implantation.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879705 | PMC |
| http://dx.doi.org/10.1007/s13770-019-00210-1 | DOI Listing |
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1Department of Otolaryngology-Head and Neck Surgery, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 06591 Republic of Korea.
Article Synopsis
- The study developed a complex using human nasal septal cartilage (hNC) and polycaprolactone (PCL) for potential transplantation into cartilage defects.
- The research involved isolating hNC from patients and testing the cell-PCL complex for cell viability, growth, and protein expression relevant to cartilage health.
- Results indicated that the hNC-PCL complex maintained high viability and expressed important cartilage proteins, suggesting it could effectively repair cartilage injuries in clinical settings.
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