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Exploration of the genetic landscape of bacterial dsDNA viruses reveals an ANI gap amid extensive mosaicism.
mSystems
January 2025
Malopolska Centre of Biotechnology, Jagiellonian University, Kraków, Poland.
Average nucleotide identity (ANI) is a widely used metric to estimate genetic relatedness, especially in microbial species delineation. While ANI calculation has been well optimized for bacteria and closely related viral genomes, accurate estimation of ANI below 80%, particularly in large reference data sets, has been challenging due to a lack of accurate and scalable methods. To bridge this gap, we introduce MANIAC, an efficient computational pipeline optimized for estimating ANI and alignment fraction (AF) in viral genomes with divergence around ANI of 70%.
View Article and Find Full Text PDFCorrelates of Susceptibility to Nicotine Pouch Use Among Australian Adolescents and Younger Adults.
Nicotine Tob Res
January 2025
Melbourne Centre for Behaviour Change, Melbourne School of Psychological Sciences, University of Melbourne, Parkville, Australia.
Introduction: Nicotine pouches are growing rapidly in popularity. These products have been found to contain high levels of nicotine, raising concerns about the risk of nicotine dependence and addiction. Preventing uptake of nicotine pouches, particularly among adolescents and younger adults, is thus important.
View Article and Find Full Text PDFAssessment of safety and effectiveness after percutaneous closure for decannulation of Veno-Arterial Extracorporeal Membrane Oxygenation: A systematic review and meta-analysis.
J Vasc Access
January 2025
RISE@Health, Departamento de Biomedicina - Unidade de Anatomia, Faculdade de Medicina, Universidade do Porto, Porto, Portugal.
Introduction: Veno-Arterial Extracorporeal Membrane Oxygenation (VA-ECMO) has emerged as a crucial component of critical care medicine, mainly as a lifesaving intervention for patients experiencing refractory cardiac arrest and respiratory failure.
Background: In the past, VA-ECMO decannulation was surgical and often associated with a high rate of periprocedural complications, such as surgical site infection, bleeding, and patient mobilization costs. To reduce the rate of these adverse events, many percutaneous techniques utilizing suture-mediated closing devices have been adopted.
Quantifying Bone Collagen Fingerprint Variation Between Species.
Mol Ecol Resour
January 2025
Manchester Institute of Biotechnology, School of Natural Sciences, University of Manchester, Manchester, UK.
Collagen is the most ubiquitous protein in the animal kingdom and one of the most abundant proteins on Earth. Despite having a relatively repetitive amino acid sequence motif that enables its triple helical structure, in type 1 collagen, that dominates skin and bone, there is enough variation for its increasing use for the biomolecular species identification of animal tissues processed or degraded beyond the amenability of DNA-based analyses. In recent years, this has been most commonly achieved through the technique of collagen peptide mass fingerprinting (PMF) known as ZooMS (Zooarchaeology by Mass Spectrometry), applied to the analysis of tens of thousands of samples across over one hundred studies in the past decade alone.
View Article and Find Full Text PDFUrinary Endotrophin and Long-term Outcomes in Kidney Transplant Recipients.
Transplant Direct
March 2024
Department of Nephrology, Odense University Hospital, Odense, Denmark.
Background: Kidney fibrosis is a suggested cause of kidney failure and premature mortality. Because collagen type VI is closely linked to kidney fibrosis, we aimed to evaluate whether urinary endotrophin, a collagen type VI fragment, is associated with graft failure and mortality among kidney transplant recipients (KTR).
Methods: In this prospective cohort study, KTR with a functioning graft ≥1-y posttransplantation were recruited; 24-h urinary endotrophin excretion was measured using an ELISA method.
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